Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_14505 and PS417_14510 are separated by 1 nucleotidesPS417_14510 and PS417_14515 overlap by 4 nucleotidesPS417_14515 and PS417_14520 overlap by 4 nucleotidesPS417_14520 and PS417_14525 overlap by 4 nucleotides PS417_14505: PS417_14505 - glycogen debranching protein, at 3,171,398 to 3,173,557 _14505 PS417_14510: PS417_14510 - acyl-CoA dehydrogenase, at 3,173,559 to 3,174,557 _14510 PS417_14515: PS417_14515 - acetylglucosaminylphosphatidylinositol deacetylase, at 3,174,554 to 3,175,312 _14515 PS417_14520: PS417_14520 - methyltransferase, at 3,175,309 to 3,175,908 _14520 PS417_14525: PS417_14525 - glycosyl transferase, at 3,175,905 to 3,176,564 _14525
Group Condition PS417_14505 PS417_14510 PS417_14515 PS417_14520 PS417_14525
seeds Growth on radish seeds for 72 hours -0.9 N.D. -2.6 +0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 N.D. -1.1 +0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 N.D. -0.7 -0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 N.D. -1.0 -0.4 N.D.
stress Choline Lysine 25 mM -0.4 N.D. -1.0 -0.4 N.D.
phage P. simiae OR Antrim MOI 0.1 +0.1 N.D. -1.9 +0.0 N.D.
nitrogen source Putrescine (N) -0.4 N.D. -1.1 -0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 N.D. -2.2 +0.2 N.D.
root root sample 6; outgrowth in LB -0.7 N.D. +0.1 -1.0 N.D.
motility inner cut, LB soft agar motility assay -0.2 N.D. -1.6 +0.2 N.D.
soil soil sample 3; outgrowth in LB -0.5 N.D. -1.2 +0.3 N.D.
phage P. simiae ORA MOI 0.1 +0.5 N.D. -1.9 -0.0 N.D.
phage JP1 MOI 1 +0.1 N.D. -1.6 +0.2 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 N.D. -0.8 -0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.2 N.D. -1.3 +0.3 N.D.
phage P. simiae Grant Run MOI 10 +0.0 N.D. -1.5 +0.4 N.D.
carbon source D-Galacturonic Acid (C) +0.3 N.D. -1.5 +0.1 N.D.
soil soil sample 2; outgrowth in LB -0.2 N.D. -1.2 +0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 N.D. -1.6 -0.1 N.D.
phage FRSphage +0.3 N.D. -1.4 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. -0.8 -0.5 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.5 N.D. -1.0 +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.1 N.D. +1.2 +0.2 N.D.
phage P. simiae Grant Run MOI 0.1 +0.9 N.D. -0.7 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.1 N.D. +1.2 -0.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 N.D. +0.7 -0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 N.D. +2.4 -0.6 N.D.
phage JP1 MOI 0.1 +0.4 N.D. +1.0 +0.3 N.D.
supernatant control Vogels_fungal_media 0.1X +0.8 N.D. +0.8 +0.2 N.D.
phage P. simiae OR1 MOI 0.1 +0.3 N.D. +1.0 +0.6 N.D.
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