Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_14410 and PS417_14415 overlap by 14 nucleotidesPS417_14415 and PS417_14420 overlap by 4 nucleotidesPS417_14420 and PS417_14425 are separated by 34 nucleotidesPS417_14425 and PS417_14430 are separated by 102 nucleotides PS417_14410: PS417_14410 - ABC transporter substrate-binding protein, at 3,149,993 to 3,151,192 _14410 PS417_14415: PS417_14415 - ABC transporter ATP-binding protein, at 3,151,179 to 3,151,982 _14415 PS417_14420: PS417_14420 - acyl-CoA dehydrogenase, at 3,151,979 to 3,153,040 _14420 PS417_14425: PS417_14425 - alkylhydroperoxidase, at 3,153,075 to 3,153,635 _14425 PS417_14430: PS417_14430 - AraC family transcriptional regulator, at 3,153,738 to 3,154,643 _14430
Group Condition PS417_14410 PS417_14415 PS417_14420 PS417_14425 PS417_14430
phage P. simiae OR1 MOI 0.1 +0.4 N.D. -0.4 -1.8 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 N.D. -1.0 -1.0 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. -0.1 -1.1 -0.2
seeds Growth on radish seeds for 72 hours +0.5 N.D. -1.0 -0.6 -0.6
seeds Growth on radish seeds for 72 hours +0.3 N.D. -0.3 -1.3 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 N.D. -0.2 -1.0 -0.4
rhizosphere rhizosphere sample 1; outgrowth in LB -0.4 N.D. +0.1 -0.5 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -1.0 N.D. -0.3 -0.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.2 N.D. +0.2 -0.8 -0.4
seeds Growth on radish seeds for 24 hours +0.6 N.D. -0.3 -1.7 +0.2
carbon source L-Valine (C) -0.1 N.D. -1.2 -0.1 +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.8 N.D. -0.3 -1.2 +1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 N.D. -0.7 -0.6 +0.5
carbon source L-Valine (C) +0.5 N.D. +0.5 -1.3 +0.0
stress Doxycycline hyclate 0.001 mg/ml +0.6 N.D. -0.5 +0.5 -0.4
phage P. simiae ORA MOI 1 +0.2 N.D. +0.4 -1.0 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 N.D. +0.6 -0.8 -0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 N.D. +0.5 -0.4 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 N.D. +1.0 -0.7 -0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. +0.0 +1.2 -0.3
seeds Growth on radish seeds for 72 hours +0.6 N.D. -0.7 +0.6 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. -0.6 +0.8 +0.6
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.7 N.D. +0.4 -0.4 +0.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 N.D. -0.0 +1.5 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 N.D. +0.5 +0.3 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. -0.2 +0.7 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.7 N.D. +0.3 +0.0 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 N.D. +0.5 +0.1 +0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 N.D. +0.4 +0.7 +1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 N.D. +2.4 +0.2 +0.0
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