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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_14335 and PS417_14340 are separated by 122 nucleotides
PS417_14340 and PS417_14345 are separated by 338 nucleotides
PS417_14345 and PS417_14350 are separated by 75 nucleotides
PS417_14350 and PS417_14355 are separated by 30 nucleotides
PS417_14335: PS417_14335 - lysine transporter LysE, at 3,137,352 to 3,137,960
_14335
PS417_14340: PS417_14340 - hypothetical protein, at 3,138,083 to 3,138,286
_14340
PS417_14345: PS417_14345 - LysR family transcriptional regulator, at 3,138,625 to 3,139,530
_14345
PS417_14350: PS417_14350 - multidrug DMT transporter permease, at 3,139,606 to 3,140,433
_14350
PS417_14355: PS417_14355 - sugar dehydrogenase, at 3,140,464 to 3,141,225
_14355
Group
Condition
PS417
_14335
PS417
_14340
PS417
_14345
PS417
_14350
PS417
_14355
stress
Phosphomycin 0.003 mg/ml
+0.2
N.D.
-0.8
-1.7
-0.2
stress
Phosphomycin 0.002 mg/ml
+0.4
N.D.
-1.1
-1.6
-0.2
stress
Phosphomycin 0.001 mg/ml
+0.2
N.D.
-0.8
-1.1
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
-0.3
N.D.
-0.3
-0.3
-0.4
phage
JP1 MOI 0.1
+0.2
N.D.
-0.4
+0.0
-0.9
motility
inner cut, LB soft agar motility assay
+0.3
N.D.
-0.4
-0.3
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
N.D.
-0.7
-0.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.3
N.D.
+0.1
-0.7
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.1
N.D.
+0.3
+0.2
-0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
N.D.
-0.4
-0.2
+0.5
agar plate interaction control
Taped volatile agar plate with no fungus
-0.4
N.D.
-0.5
+0.6
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
N.D.
-0.1
+0.3
-0.6
phage
JP1 MOI 1
+0.1
N.D.
-0.7
-0.1
+0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
N.D.
-0.1
-0.5
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
N.D.
-0.2
-0.5
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
N.D.
-0.6
-0.1
+0.3
phage
P. simiae OR Antrim MOI 1
+0.1
N.D.
+0.6
-0.4
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.2
N.D.
-0.4
+0.6
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
N.D.
+0.4
-0.3
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
N.D.
+0.1
-0.3
+0.5
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.2
N.D.
+0.2
+0.5
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.1
N.D.
+0.3
-0.3
+0.5
stress
methylglyoxal 0.032 vol%
-0.3
N.D.
+1.2
-0.0
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.3
N.D.
+1.4
-0.0
+0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.0
N.D.
+0.2
+0.4
+0.6
motility
outer cut, LB soft agar motility assay
+0.2
N.D.
+0.6
+0.1
+0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
N.D.
+1.1
+0.2
-0.1
seeds
Growth on radish seeds for 72 hours
-0.1
N.D.
+1.8
-0.2
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.3
N.D.
+0.2
+0.7
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
N.D.
+0.2
+0.5
+0.8
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