Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_14295 and PS417_14300 are separated by 187 nucleotidesPS417_14300 and PS417_14305 are separated by 14 nucleotidesPS417_14305 and PS417_14310 are separated by 42 nucleotidesPS417_14310 and PS417_14315 are separated by 61 nucleotides PS417_14295: PS417_14295 - AraC family transcriptional regulator, at 3,128,638 to 3,129,396 _14295 PS417_14300: PS417_14300 - aspartate:proton symporter, at 3,129,584 to 3,131,203 _14300 PS417_14305: PS417_14305 - hydroxyproline-2-epimerase, at 3,131,218 to 3,132,144 _14305 PS417_14310: PS417_14310 - dihydrodipicolinate synthetase, at 3,132,187 to 3,133,134 _14310 PS417_14315: PS417_14315 - 2,5-dioxovalerate dehydrogenase, at 3,133,196 to 3,134,767 _14315
Group Condition PS417_14295 PS417_14300 PS417_14305 PS417_14310 PS417_14315
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.5 -0.9 -0.0 -2.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -0.0 -0.9 -0.6 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.5 -0.3 -0.5 -0.0 -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.1 -1.6 +0.1 -0.6
carbon source Carbon source L-Citrulline 10 mM -0.6 +0.1 -1.1 -0.3 -0.5
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.0 -0.3 -0.6 -0.4 -1.0
phage JP1 MOI 0.1 +0.2 -0.1 +0.1 -0.0 -2.3
phage JP1 MOI 10 +0.6 -0.5 -2.4 -0.0 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.1 -0.5 +0.0 -1.5
root root sample 6; outgrowth in LB -1.7 -0.2 -0.6 -0.0 +0.7
nophagecontrol Only library -1.3 -0.1 +0.5 -0.5 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 +0.0 -1.2 -0.5 +0.8
rhizosphere rhizosphere sample 2; outgrowth in LB -0.7 +0.3 +0.5 +0.0 -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 +0.1 -0.5 -0.2 -1.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.2 +0.7 -0.7 -1.0
no phage control Time6-nophage -0.9 +0.2 -0.7 -0.1 +0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 +0.1 -0.9 -0.2 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 +0.1 -1.1 +0.3 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.4 -1.0 +0.2 +0.9
phage P. simiae ORA MOI 10 +0.8 +0.4 -0.9 -0.1 -0.4
phage JP1 MOI 10 +0.5 +0.2 +0.7 -0.1 -1.4
rhizosphere rhizosphere sample 8; outgrowth in LB +0.9 -0.2 -1.0 +0.3 +0.4
stress Gentamicin 0.008 mg/ml -0.4 -0.0 -0.6 +0.3 +1.1
phage P. simiae OR1 MOI 0.1 +1.2 -0.3 -0.2 +0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.3 +0.2 -1.0 +0.5 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.0 +0.7 +0.5 -0.5 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +1.3 +0.7 -0.3 +0.2
seeds Growth on radish seeds for 72 hours +1.0 -0.0 -0.2 -0.2 +1.1
carbon source L-Valine (C) -0.1 +0.3 +0.7 +0.0 +1.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 +0.9 +0.6 -0.1 +1.9
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