Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_14140 and PS417_14145 are separated by 146 nucleotidesPS417_14145 and PS417_14150 are separated by 170 nucleotidesPS417_14150 and PS417_14155 overlap by 4 nucleotidesPS417_14155 and PS417_14160 are separated by 71 nucleotides PS417_14140: PS417_14140 - membrane protein, at 3,102,189 to 3,102,893 _14140 PS417_14145: PS417_14145 - hypothetical protein, at 3,103,040 to 3,103,393 _14145 PS417_14150: PS417_14150 - branched-chain amino acid ABC transporter, at 3,103,564 to 3,103,875 _14150 PS417_14155: PS417_14155 - branched-chain amino acid ABC transporter permease, at 3,103,872 to 3,104,567 _14155 PS417_14160: PS417_14160 - hypothetical protein, at 3,104,639 to 3,104,854 _14160
Group Condition PS417_14140 PS417_14145 PS417_14150 PS417_14155 PS417_14160
rhizosphere rhizosphere sample 2; outgrowth in LB -3.5 +0.2 N.D. -0.3 -0.2
phage JP1 MOI 10 -0.8 -0.4 N.D. -0.8 -0.5
phage P. simiae Grant Run MOI 10 -0.5 +0.2 N.D. -0.6 -1.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.8 -0.0 N.D. +0.1 -0.4
phage JP1 MOI 1 +0.0 -0.8 N.D. -0.9 -0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.0 -0.4 N.D. -0.4 -0.3
supernatant RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -1.4 -0.2 N.D. -0.2 -0.2
phage P. simiae ORA MOI 0.1 -0.1 -0.3 N.D. -0.4 -1.1
seeds Growth on radish seeds for 72 hours -1.0 -0.5 N.D. -0.0 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.7 +0.2 N.D. +0.2 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 +0.2 N.D. -0.7 -0.3
solid stress 80% Methanol 0.5 mM; solid stress -1.2 -0.2 N.D. +0.1 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.3 +0.2 N.D. -0.7 +0.2
seeds Growth on radish seeds for 24 hours -0.9 +0.5 N.D. -1.1 +0.0
phage P. simiae Grant Run MOI 10 +0.3 -0.3 N.D. -0.9 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.6 +0.7 N.D. -0.5 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.9 -0.7 N.D. +0.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 +0.2 N.D. -1.0 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.8 +0.1 N.D. +0.9 -0.1
supernatant control Vogels_fungal_media 0.1X -0.8 +0.4 N.D. +0.3 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.4 +0.5 N.D. -0.1 +0.3
seeds Growth on radish seeds for 72 hours -1.5 +0.1 N.D. -0.2 +1.2
phage Wcs_1 +0.8 -0.4 N.D. -1.0 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 +0.2 N.D. -1.0 -0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.9 +0.4 N.D. +0.1 +0.6
phage FRSphage +1.0 -0.6 N.D. -0.3 +0.1
phage Wcs_0.1 +0.8 -0.2 N.D. -0.7 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.2 +1.2 N.D. +0.1 +0.4
agar plate interaction control Taped volatile agar plate with no fungus +1.2 -0.5 N.D. +0.0 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.0 +0.5 N.D. +0.1 +0.9
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