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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13850 and PS417_13855 are separated by 9 nucleotides
PS417_13855 and PS417_13860 are separated by 12 nucleotides
PS417_13860 and PS417_13865 are separated by 200 nucleotides
PS417_13865 and PS417_13870 are separated by 70 nucleotides
PS417_13850: PS417_13850 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5)
(from data)
, at 3,042,450 to 3,043,577
_13850
PS417_13855: PS417_13855 - acetyl-CoA acetyltransferase, at 3,043,587 to 3,044,771
_13855
PS417_13860: PS417_13860 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 3,044,784 to 3,045,551
_13860
PS417_13865: PS417_13865 - bestrophin, at 3,045,752 to 3,046,645
_13865
PS417_13870: PS417_13870 - hypothetical protein, at 3,046,716 to 3,047,774
_13870
Group
Condition
PS417
_13850
PS417
_13855
PS417
_13860
PS417
_13865
PS417
_13870
carbon source
L-Isoleucine (C)
-5.7
-4.5
N.D.
+0.4
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.7
-4.0
N.D.
-0.2
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.8
-2.2
N.D.
-0.3
-0.5
solid stress
Fraxetin 3 mM; solid stress
+0.3
+0.2
N.D.
-0.0
-5.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-1.5
N.D.
-0.7
-2.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.2
N.D.
-0.2
-3.4
solid stress
Fraxetin 3 mM; solid stress
+0.3
+0.7
N.D.
+0.3
-4.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.4
N.D.
-0.5
-2.8
phage
JP1 MOI 1
+0.3
-2.2
N.D.
+0.1
-1.4
exudates
RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x
-0.3
-1.3
N.D.
-0.5
-1.1
supernatant control
Vogels_fungal_media 0.1X
-0.4
-2.5
N.D.
-0.4
+0.2
phage
JP1 MOI 1
+0.3
-2.9
N.D.
-0.2
-0.3
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.3
-1.1
N.D.
+0.2
-1.7
solid stress
Fraxetin 3 mM; solid stress
-0.2
-0.1
N.D.
-0.4
-1.8
carbon source
L-Valine (C)
-0.4
-1.1
N.D.
-0.1
-0.9
carbon source
Carbon source D-Mannitol 5 mM
-0.4
-1.6
N.D.
+0.1
-0.6
seeds
Growth on radish seeds for 24 hours
+0.5
-2.1
N.D.
-0.2
-0.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.4
-2.5
N.D.
+0.2
+0.4
solid stress
Fraxetin 2 mM; solid stress
+0.0
+0.8
N.D.
-0.1
-2.6
carbon source
L-Valine (C)
-0.8
-1.8
N.D.
+0.2
+0.5
phage
P. simiae OR1 MOI 0.1
-0.8
+0.4
N.D.
-0.3
-1.0
solid stress
Fraxetin 3 mM; solid stress
+0.1
+0.4
N.D.
-0.3
-1.9
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.5
-1.3
N.D.
+0.9
-0.7
root
root sample 6; outgrowth in LB
+0.4
-1.0
N.D.
+0.4
-1.3
phage
P. simiae OR Antrim MOI 1
-0.0
+0.8
N.D.
-0.8
-1.2
solid stress
Fraxetin 2 mM; solid stress
+0.6
-0.8
N.D.
+0.2
-1.3
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.4
-0.3
N.D.
+0.6
-1.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+1.0
N.D.
-0.4
-1.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.2
+1.1
N.D.
+0.6
-1.4
carbon source
2'-Deoxyinosine 5 mM (C)
-0.0
+2.4
N.D.
+0.1
+0.2
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