Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13850 and PS417_13855 are separated by 9 nucleotidesPS417_13855 and PS417_13860 are separated by 12 nucleotidesPS417_13860 and PS417_13865 are separated by 200 nucleotidesPS417_13865 and PS417_13870 are separated by 70 nucleotides PS417_13850: PS417_13850 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data), at 3,042,450 to 3,043,577 _13850 PS417_13855: PS417_13855 - acetyl-CoA acetyltransferase, at 3,043,587 to 3,044,771 _13855 PS417_13860: PS417_13860 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 3,044,784 to 3,045,551 _13860 PS417_13865: PS417_13865 - bestrophin, at 3,045,752 to 3,046,645 _13865 PS417_13870: PS417_13870 - hypothetical protein, at 3,046,716 to 3,047,774 _13870
Group Condition PS417_13850 PS417_13855 PS417_13860 PS417_13865 PS417_13870
carbon source L-Isoleucine (C) -5.7 -4.5 N.D. +0.4 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.7 -4.0 N.D. -0.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.8 -2.2 N.D. -0.3 -0.5
solid stress Fraxetin 3 mM; solid stress +0.3 +0.2 N.D. -0.0 -5.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -1.5 N.D. -0.7 -2.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.2 N.D. -0.2 -3.4
solid stress Fraxetin 3 mM; solid stress +0.3 +0.7 N.D. +0.3 -4.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.4 N.D. -0.5 -2.8
phage JP1 MOI 1 +0.3 -2.2 N.D. +0.1 -1.4
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x -0.3 -1.3 N.D. -0.5 -1.1
supernatant control Vogels_fungal_media 0.1X -0.4 -2.5 N.D. -0.4 +0.2
phage JP1 MOI 1 +0.3 -2.9 N.D. -0.2 -0.3
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.3 -1.1 N.D. +0.2 -1.7
solid stress Fraxetin 3 mM; solid stress -0.2 -0.1 N.D. -0.4 -1.8
carbon source L-Valine (C) -0.4 -1.1 N.D. -0.1 -0.9
carbon source Carbon source D-Mannitol 5 mM -0.4 -1.6 N.D. +0.1 -0.6
seeds Growth on radish seeds for 24 hours +0.5 -2.1 N.D. -0.2 -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.4 -2.5 N.D. +0.2 +0.4
solid stress Fraxetin 2 mM; solid stress +0.0 +0.8 N.D. -0.1 -2.6
carbon source L-Valine (C) -0.8 -1.8 N.D. +0.2 +0.5
phage P. simiae OR1 MOI 0.1 -0.8 +0.4 N.D. -0.3 -1.0
solid stress Fraxetin 3 mM; solid stress +0.1 +0.4 N.D. -0.3 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 -1.3 N.D. +0.9 -0.7
root root sample 6; outgrowth in LB +0.4 -1.0 N.D. +0.4 -1.3
phage P. simiae OR Antrim MOI 1 -0.0 +0.8 N.D. -0.8 -1.2
solid stress Fraxetin 2 mM; solid stress +0.6 -0.8 N.D. +0.2 -1.3
rhizosphere rhizosphere sample 1; outgrowth in LB +0.4 -0.3 N.D. +0.6 -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +1.0 N.D. -0.4 -1.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 +1.1 N.D. +0.6 -1.4
carbon source 2'-Deoxyinosine 5 mM (C) -0.0 +2.4 N.D. +0.1 +0.2
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