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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13840 and PS417_13845 are separated by 61 nucleotides
PS417_13845 and PS417_13850 are separated by 11 nucleotides
PS417_13850 and PS417_13855 are separated by 9 nucleotides
PS417_13855 and PS417_13860 are separated by 12 nucleotides
PS417_13840: PS417_13840 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5)
(from data)
, at 3,040,452 to 3,041,603
_13840
PS417_13845: PS417_13845 - enoyl-CoA hydratase, at 3,041,665 to 3,042,438
_13845
PS417_13850: PS417_13850 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5)
(from data)
, at 3,042,450 to 3,043,577
_13850
PS417_13855: PS417_13855 - acetyl-CoA acetyltransferase, at 3,043,587 to 3,044,771
_13855
PS417_13860: PS417_13860 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 3,044,784 to 3,045,551
_13860
Group
Condition
PS417
_13840
PS417
_13845
PS417
_13850
PS417
_13855
PS417
_13860
carbon source
L-Isoleucine (C)
-0.4
N.D.
-5.7
-4.5
N.D.
carbon source
L-Valine (C)
-3.3
N.D.
-0.8
-1.8
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
N.D.
-1.7
-4.0
N.D.
carbon source
L-Valine (C)
-3.1
N.D.
-0.4
-1.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
-1.8
-2.2
N.D.
supernatant control
Vogels_fungal_media 0.1X
-0.1
N.D.
-0.4
-2.5
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.0
N.D.
-0.4
-2.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
N.D.
+0.1
-1.3
N.D.
phage
JP1 MOI 1
-0.1
N.D.
+0.3
-2.9
N.D.
phage
Wcs_1
-0.6
N.D.
-0.1
-1.7
N.D.
carbon source
Carbon source D-Mannitol 5 mM
-0.2
N.D.
-0.4
-1.6
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.1
N.D.
-0.5
-1.3
N.D.
phage
JP1 MOI 1
+0.5
N.D.
+0.3
-2.2
N.D.
motility_chemotaxis
Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.4
N.D.
+0.5
-1.5
N.D.
seeds
Growth on radish seeds for 24 hours
+0.4
N.D.
+0.5
-2.1
N.D.
exudates
RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x
+0.4
N.D.
-0.3
-1.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
N.D.
+0.3
-1.5
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
+0.7
N.D.
-0.4
-1.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
-0.1
-1.2
N.D.
soil
soil sample 3; outgrowth in LB
-0.6
N.D.
-0.8
+0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
+0.6
-0.9
N.D.
phage
P. simiae OR1 MOI 0.1
+0.7
N.D.
-0.8
+0.4
N.D.
phage
JP1 MOI 10
+0.3
N.D.
-0.9
+0.9
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.2
N.D.
-0.6
+1.1
N.D.
motility
inner cut, LB soft agar motility assay
+0.5
N.D.
-0.4
+0.9
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
N.D.
+0.2
+1.2
N.D.
seeds
Growth on radish seeds for 72 hours
+0.3
N.D.
+0.3
+1.3
N.D.
phage
JP1 MOI 0.1
+0.5
N.D.
+0.2
+1.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
N.D.
+0.0
+1.4
N.D.
carbon source
2'-Deoxyinosine 5 mM (C)
+0.0
N.D.
-0.0
+2.4
N.D.
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