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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_01365 and PS417_01370 are separated by 3 nucleotides
PS417_01370 and PS417_01375 are separated by 14 nucleotides
PS417_01375 and PS417_01380 are separated by 37 nucleotides
PS417_01380 and PS417_01385 are separated by 519 nucleotides
PS417_01365: PS417_01365 - 3-oxoacyl-ACP reductase, at 297,836 to 298,537
_01365
PS417_01370: PS417_01370 - peptidase M48, at 298,541 to 300,118
_01370
PS417_01375: PS417_01375 - hypothetical protein, at 300,133 to 300,498
_01375
PS417_01380: PS417_01380 - nucleotide cyclase, at 300,536 to 301,876
_01380
PS417_01385: PS417_01385 - NAD-dependent dehydratase, at 302,396 to 303,328
_01385
Group
Condition
PS417
_01365
PS417
_01370
PS417
_01375
PS417
_01380
PS417
_01385
phage
P. simiae ORA MOI 1
N.D.
-0.4
-0.9
-0.4
-0.5
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.2
-0.6
-0.8
-0.3
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
+0.2
-1.3
-0.5
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.1
-1.0
+0.2
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.1
-1.0
+0.4
-0.6
phage
JP1 MOI 0.1
N.D.
+0.2
-1.0
+0.4
-0.4
phage
P. simiae OR Antrim MOI 10
N.D.
-0.1
-0.5
+0.8
-0.6
seeds
Growth on radish seeds for 24 hours
N.D.
+0.0
+0.9
-0.4
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.3
-0.4
+0.7
-0.5
phage
P. simiae OR Antrim MOI 10
N.D.
+0.0
+0.8
+0.4
-0.9
phage
P. simiae OR Antrim MOI 1
N.D.
+0.0
+0.6
+0.5
-0.7
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.3
+0.6
-0.8
+0.4
root
root sample 6; outgrowth in LB
N.D.
+0.5
+1.0
-0.3
-0.7
phage
P. simiae OR Antrim MOI 1
N.D.
-0.2
+0.9
+0.6
-0.6
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-0.4
+1.2
+0.1
-0.2
soil
soil sample 2; outgrowth in LB
N.D.
+0.1
+1.0
-0.5
+0.0
seeds
Growth on radish seeds for 72 hours
N.D.
-0.2
+0.7
+0.6
-0.2
phage
P. simiae OR Antrim MOI 0.1
N.D.
+0.3
+0.4
+0.6
-0.4
carbon source
L-Valine (C)
N.D.
+0.3
-0.5
+0.6
+0.6
phage
JP1 MOI 1
N.D.
-0.3
+0.6
+0.4
+0.3
phage
P. simiae Grant Run MOI 0.1
N.D.
+0.2
+1.0
+0.5
-0.6
stress
Dimethyl Sulfoxide 5 vol%
N.D.
-0.1
+0.2
-0.4
+1.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.2
+0.3
+0.6
+0.8
liquid stress
Fraxetin 2 mM; liquid stress
N.D.
-0.1
+0.5
+0.4
+0.8
liquid stress
80% Methanol 2 mM; liquid stress
N.D.
-0.1
+0.5
+0.4
+0.9
stress
Choline chloride 300 mM
N.D.
+0.0
+0.1
+0.2
+1.4
soil
soil sample 8; outgrowth in LB
N.D.
+0.4
+0.2
+0.6
+0.6
soil
soil sample 6; outgrowth in LB
N.D.
+0.4
+0.8
+0.7
+0.0
seeds
Growth on radish seeds for 72 hours
N.D.
-0.3
+1.5
+1.1
-0.5
stress
LB_noSalt with Chloride 500 mM
N.D.
+0.2
+0.6
+0.2
+0.9
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