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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_00130 and PS417_00135 are separated by 133 nucleotides
PS417_00135 and PS417_00140 are separated by 15 nucleotides
PS417_00140 and PS417_00145 are separated by 103 nucleotides
PS417_00145 and PS417_00150 are separated by 31 nucleotides
PS417_00130: PS417_00130 - SulP family inorganic anion transporter, at 27,295 to 28,887
_00130
PS417_00135: PS417_00135 - glycine/betaine ABC transporter substrate-binding protein, at 29,021 to 29,935
_00135
PS417_00140: PS417_00140 - choline-sulfatase, at 29,951 to 31,465
_00140
PS417_00145: PS417_00145 - LysR family transcriptional regulator, at 31,569 to 32,519
_00145
PS417_00150: PS417_00150 - 4,5-dioxygenase, at 32,551 to 32,880
_00150
Group
Condition
PS417
_00130
PS417
_00135
PS417
_00140
PS417
_00145
PS417
_00150
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.8
-0.0
-0.1
-0.2
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.3
-0.2
-0.3
-0.5
phage
JP1 MOI 1
-0.8
-0.3
+0.1
-0.3
-0.1
carbon source
D-Galactose (C)
-0.1
+0.0
-0.1
-0.4
-0.8
soil
soil sample 8; outgrowth in LB
-0.4
-0.1
-0.2
-0.0
-0.5
soil
soil sample 7; outgrowth in LB
-0.1
-0.5
-0.1
-0.3
-0.3
motility
inner cut, LB soft agar motility assay
-0.4
-0.5
+0.1
-0.3
-0.3
carbon source
D-Galactose (C)
-0.4
+0.0
+0.1
-0.2
-0.8
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.2
-0.1
+0.1
-0.0
-0.9
soil
soil sample 4; outgrowth in LB
-0.6
-0.3
-0.3
+0.0
+0.2
phage
JP1 MOI 10
-0.2
-0.5
+0.1
+0.2
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
+0.4
-0.4
-0.2
-0.1
carbon source
L-Valine (C)
-0.4
-0.2
-0.0
-0.4
+0.4
supernatant control
Vogels_fungal_media 0.1X
-0.4
+0.2
+0.2
+0.0
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
-0.4
+0.1
+0.0
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.6
-0.4
-0.3
-0.6
agar plate interaction control
Taped volatile agar plate with no fungus
+0.6
-0.2
-0.2
+0.1
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.7
-0.3
-0.0
+0.1
-0.5
phage
P. simiae Grant Run MOI 0.1
-0.5
-0.2
+0.3
+0.6
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.2
-0.4
+0.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.1
-0.2
-0.1
+0.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
+0.1
+0.2
-0.5
+0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
-0.2
-0.2
+0.4
+0.0
stress
methylglyoxal 0.032 vol%
+0.9
-0.2
-0.3
-0.1
+0.6
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.3
+0.1
+0.1
+0.1
+0.9
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.9
-0.1
-0.1
+0.5
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.9
+0.4
-0.1
-0.5
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.4
+0.0
+0.3
+0.6
+0.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+1.6
+0.1
-0.3
-0.0
+0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.5
+0.4
-0.2
-0.0
+0.8
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