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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13675 and PS417_13680 are separated by 65 nucleotides
PS417_13680 and PS417_13685 are separated by 109 nucleotides
PS417_13685 and PS417_13690 are separated by 114 nucleotides
PS417_13690 and PS417_13695 are separated by 110 nucleotides
PS417_13675: PS417_13675 - endoribonuclease L-PSP, at 3,009,783 to 3,010,163
_13675
PS417_13680: PS417_13680 - 3-oxoacyl-ACP synthase, at 3,010,229 to 3,011,497
_13680
PS417_13685: PS417_13685 - HxlR family transcriptional regulator, at 3,011,607 to 3,012,095
_13685
PS417_13690: PS417_13690 - chemotaxis protein, at 3,012,210 to 3,013,565
_13690
PS417_13695: PS417_13695 - porin, at 3,013,676 to 3,014,998
_13695
Group
Condition
PS417
_13675
PS417
_13680
PS417
_13685
PS417
_13690
PS417
_13695
carbon source
L-Valine (C)
-0.2
-1.4
-0.3
+0.0
-0.5
phage
JP1 MOI 10
-0.8
-0.4
-0.3
+0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
-0.3
-0.6
+0.1
+0.4
motility
inner cut, LB soft agar motility assay
-0.6
-0.5
-0.1
-0.1
+0.2
root
root sample 6; outgrowth in LB
+0.5
-0.3
+0.8
-2.0
-0.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.7
+0.5
-0.5
-0.2
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.2
-0.8
-0.5
-0.1
phage
P. simiae OR Antrim MOI 10
+0.5
-0.3
-0.5
-0.3
-0.1
supernatant
RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 and Trichoderma harzanium TH1 and Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-0.5
-0.1
+0.5
-0.4
-0.1
agar plate interaction control
Taped volatile agar plate with no fungus
-0.6
+0.3
-0.6
+0.3
+0.0
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-1.1
-0.1
+0.4
+0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-0.0
-0.4
-0.3
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
-0.3
-0.6
+0.0
-0.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.1
+0.4
+0.3
-1.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.5
-0.1
+0.5
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-0.2
-0.7
-0.0
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.5
-0.4
+0.9
+0.1
-0.0
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.2
-0.1
+0.4
-0.5
+0.2
soil
soil sample 8; outgrowth in LB
-0.1
+0.4
-0.5
+0.5
-0.1
exudates
RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x
-0.1
+0.3
+0.7
-0.4
-0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
+0.2
+0.1
+0.6
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
-0.1
-0.4
+0.5
+0.0
soil
soil sample 7; outgrowth in LB
-0.3
+0.4
+0.2
+0.4
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
+0.7
-0.2
+0.2
+0.2
carbon source
2'-Deoxyinosine 5 mM (C)
-0.1
+0.3
-0.3
+0.6
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.3
+0.2
+0.6
-0.3
+0.1
stress
Neomycin 0.04 mg/ml
-0.2
+0.0
+1.3
+0.2
+0.1
stress
methylglyoxal 0.032 vol%
+0.5
+0.5
-0.1
+0.3
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.2
+0.2
+0.4
+0.5
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+2.6
-0.4
-0.1
+0.1
+0.2
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