Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13660 and PS417_13665 are separated by 109 nucleotidesPS417_13665 and PS417_13670 overlap by 23 nucleotidesPS417_13670 and PS417_13675 are separated by 70 nucleotidesPS417_13675 and PS417_13680 are separated by 65 nucleotides PS417_13660: PS417_13660 - AsnC family transcriptional regulator, at 3,006,300 to 3,006,743 _13660 PS417_13665: PS417_13665 - multidrug DMT transporter permease, at 3,006,853 to 3,007,755 _13665 PS417_13670: PS417_13670 - protease, at 3,007,733 to 3,009,712 _13670 PS417_13675: PS417_13675 - endoribonuclease L-PSP, at 3,009,783 to 3,010,163 _13675 PS417_13680: PS417_13680 - 3-oxoacyl-ACP synthase, at 3,010,229 to 3,011,497 _13680
Group Condition PS417_13660 PS417_13665 PS417_13670 PS417_13675 PS417_13680
soil soil sample 5; outgrowth in LB -1.1 -0.5 -0.0 -0.6 -0.3
phage JP1 MOI 10 -1.0 +0.3 -0.5 -0.8 -0.4
soil soil sample 6; outgrowth in LB -1.1 -0.2 -0.5 -0.4 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.4 -0.7 -0.7 -0.3
stress Choline chloride 300 mM -0.6 -0.3 -0.1 -0.5 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.7 -0.1 -0.6 -0.3 +0.0
motility inner cut, LB soft agar motility assay +0.2 -0.2 -0.3 -0.6 -0.5
solid stress Fraxetin 3 mM; solid stress -1.2 +0.1 +0.1 +0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 -0.4 -0.3 -0.3 -0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.0 +0.3 -0.2 +0.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.2 -0.3 -0.8 +0.0
carbon source L-Valine (C) +0.5 +0.5 -0.1 -0.2 -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 +0.2 -0.5 +0.7 -0.3
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 +0.0 +0.2 -0.7 +0.5
r2a control R2A control +0.3 -0.4 -0.5 +0.4 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 -0.1 -0.3 +0.5 +0.4
phage P. simiae OR1 MOI 0.1 +1.1 -0.2 -0.7 -0.1 -0.3
nophagecontrol Only library -0.4 +0.6 -0.6 +0.6 -0.2
carbon source Carbon source D-Mannitol 2.5 mM -0.9 -0.1 +0.2 +0.4 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.2 +0.6 +0.3 -0.5
stress methylglyoxal 0.032 vol% +0.3 -0.5 -0.3 +0.5 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.6 +0.2 -0.4 -0.5 -0.4
phage P. simiae ORA MOI 0.1 +0.5 +0.2 -0.3 +0.5 -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 +0.6 +0.4 -0.4 +0.3
solid stress 80% Methanol 2 mM; solid stress +0.1 +0.3 +0.3 +0.8 -0.3
phage JP1 MOI 1 +0.6 +0.8 +0.1 -0.1 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.0 -0.4 -0.2 +0.3 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.5 -0.1 +0.3 +2.6 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.2 -0.0 +0.2 +0.3 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +3.0 -0.0 +0.3 -0.1 +0.0
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