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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13655 and PS417_13660 are separated by 10 nucleotides
PS417_13660 and PS417_13665 are separated by 109 nucleotides
PS417_13665 and PS417_13670 overlap by 23 nucleotides
PS417_13670 and PS417_13675 are separated by 70 nucleotides
PS417_13655: PS417_13655 - xylulose kinase, at 3,004,799 to 3,006,289
_13655
PS417_13660: PS417_13660 - AsnC family transcriptional regulator, at 3,006,300 to 3,006,743
_13660
PS417_13665: PS417_13665 - multidrug DMT transporter permease, at 3,006,853 to 3,007,755
_13665
PS417_13670: PS417_13670 - protease, at 3,007,733 to 3,009,712
_13670
PS417_13675: PS417_13675 - endoribonuclease L-PSP, at 3,009,783 to 3,010,163
_13675
Group
Condition
PS417
_13655
PS417
_13660
PS417
_13665
PS417
_13670
PS417
_13675
phage
JP1 MOI 10
+0.3
-1.0
+0.3
-0.5
-0.8
soil
soil sample 6; outgrowth in LB
+0.6
-1.1
-0.2
-0.5
-0.4
soil
soil sample 5; outgrowth in LB
+0.7
-1.1
-0.5
-0.0
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-0.5
-0.0
+0.4
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
-0.5
+0.4
-0.7
-0.7
carbon source
Carbon source D-Mannitol 2.5 mM
-0.3
-0.9
-0.1
+0.2
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.6
+0.2
-0.5
+0.7
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.3
-1.0
+0.3
-0.2
+0.1
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.7
+0.3
-0.3
-0.9
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.6
+0.5
-0.2
-0.3
-0.8
stress
Paraquat dichloride 0.008 mg/ml
-0.3
+0.6
-0.4
+0.3
-0.3
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
+0.3
+0.6
-0.4
-0.5
-0.1
phage
P. simiae Grant Run MOI 10
-0.4
+0.6
+0.3
-0.4
-0.2
nophagecontrol
Only library
-0.1
-0.4
+0.6
-0.6
+0.6
phage
P. simiae OR1 MOI 0.1
+0.3
+1.1
-0.2
-0.7
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.5
+1.6
+0.2
-0.4
-0.5
stress
methylglyoxal 0.032 vol%
+0.3
+0.3
-0.5
-0.3
+0.5
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.3
+0.2
+0.6
+0.4
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.4
+0.8
-0.2
-0.3
-0.2
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
+0.6
-0.3
-0.1
+0.7
-0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.6
-0.6
-0.0
+0.7
+0.1
rhizosphere
rhizosphere sample 1; outgrowth in LB
+1.1
-0.8
+0.2
-0.0
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.0
-0.0
+0.3
-0.2
-0.3
phage
JP1 MOI 1
-0.5
+0.6
+0.8
+0.1
-0.1
solid stress
80% Methanol 2 mM; solid stress
-0.5
+0.1
+0.3
+0.3
+0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.2
-0.5
-0.2
+0.1
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
+1.0
-0.4
-0.2
+0.3
phage
P. simiae ORA MOI 0.1
+0.5
+0.5
+0.2
-0.3
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.1
-0.5
-0.1
+0.3
+2.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.5
+3.0
-0.0
+0.3
-0.1
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