Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13655 and PS417_13660 are separated by 10 nucleotidesPS417_13660 and PS417_13665 are separated by 109 nucleotidesPS417_13665 and PS417_13670 overlap by 23 nucleotidesPS417_13670 and PS417_13675 are separated by 70 nucleotides PS417_13655: PS417_13655 - xylulose kinase, at 3,004,799 to 3,006,289 _13655 PS417_13660: PS417_13660 - AsnC family transcriptional regulator, at 3,006,300 to 3,006,743 _13660 PS417_13665: PS417_13665 - multidrug DMT transporter permease, at 3,006,853 to 3,007,755 _13665 PS417_13670: PS417_13670 - protease, at 3,007,733 to 3,009,712 _13670 PS417_13675: PS417_13675 - endoribonuclease L-PSP, at 3,009,783 to 3,010,163 _13675
Group Condition PS417_13655 PS417_13660 PS417_13665 PS417_13670 PS417_13675
phage JP1 MOI 10 +0.3 -1.0 +0.3 -0.5 -0.8
soil soil sample 6; outgrowth in LB +0.6 -1.1 -0.2 -0.5 -0.4
soil soil sample 5; outgrowth in LB +0.7 -1.1 -0.5 -0.0 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.5 -0.0 +0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 -0.5 +0.4 -0.7 -0.7
carbon source Carbon source D-Mannitol 2.5 mM -0.3 -0.9 -0.1 +0.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.6 +0.2 -0.5 +0.7
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.3 -1.0 +0.3 -0.2 +0.1
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.7 +0.3 -0.3 -0.9 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 +0.5 -0.2 -0.3 -0.8
stress Paraquat dichloride 0.008 mg/ml -0.3 +0.6 -0.4 +0.3 -0.3
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.3 +0.6 -0.4 -0.5 -0.1
phage P. simiae Grant Run MOI 10 -0.4 +0.6 +0.3 -0.4 -0.2
nophagecontrol Only library -0.1 -0.4 +0.6 -0.6 +0.6
phage P. simiae OR1 MOI 0.1 +0.3 +1.1 -0.2 -0.7 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 +1.6 +0.2 -0.4 -0.5
stress methylglyoxal 0.032 vol% +0.3 +0.3 -0.5 -0.3 +0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 +0.2 +0.6 +0.4 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.4 +0.8 -0.2 -0.3 -0.2
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI +0.6 -0.3 -0.1 +0.7 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 -0.6 -0.0 +0.7 +0.1
rhizosphere rhizosphere sample 1; outgrowth in LB +1.1 -0.8 +0.2 -0.0 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.0 -0.0 +0.3 -0.2 -0.3
phage JP1 MOI 1 -0.5 +0.6 +0.8 +0.1 -0.1
solid stress 80% Methanol 2 mM; solid stress -0.5 +0.1 +0.3 +0.3 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 -0.5 -0.2 +0.1 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 +1.0 -0.4 -0.2 +0.3
phage P. simiae ORA MOI 0.1 +0.5 +0.5 +0.2 -0.3 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.1 -0.5 -0.1 +0.3 +2.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 +3.0 -0.0 +0.3 -0.1
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