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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13625 and PS417_13635 are separated by 164 nucleotides
PS417_13635 and PS417_13640 overlap by 8 nucleotides
PS417_13640 and PS417_13645 are separated by 30 nucleotides
PS417_13645 and PS417_13650 are separated by 22 nucleotides
PS417_13625: PS417_13625 - cytochrome B561, at 2,999,595 to 3,000,137
_13625
PS417_13635: PS417_13635 - D-ribose transporter ATP-binding protein, at 3,000,302 to 3,001,834
_13635
PS417_13640: PS417_13640 - ribose ABC transporter permease, at 3,001,827 to 3,002,804
_13640
PS417_13645: PS417_13645 - ABC transporter, at 3,002,835 to 3,003,767
_13645
PS417_13650: PS417_13650 - LacI family transcriptional regulator, at 3,003,790 to 3,004,791
_13650
Group
Condition
PS417
_13625
PS417
_13635
PS417
_13640
PS417
_13645
PS417
_13650
motility
outer cut, LB soft agar motility assay
-0.3
-0.2
-0.7
-0.6
-0.4
root
root sample 6; outgrowth in LB
-0.5
+0.0
-0.8
-1.2
+0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
-0.2
-0.9
-1.2
-0.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.5
-0.3
-0.9
-0.7
-0.4
carbon source
D-Fructose (C)
+0.1
-0.9
-0.8
-0.7
+1.0
stress
methylglyoxal 0.032 vol%
+0.5
+0.1
-1.0
+0.2
-1.1
carbon source
D-Fructose (C)
+0.1
-0.6
-0.7
-1.1
+1.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.2
-0.1
-1.1
-0.7
+0.5
solid stress
Fraxetin 3 mM; solid stress
-0.3
-0.2
-0.7
+0.8
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.3
-0.6
+0.7
-0.2
seeds
Growth on radish seeds for 72 hours
-0.4
-0.2
-1.0
+0.2
+0.6
seeds
Growth on radish seeds for 24 hours
-0.3
+0.1
-1.4
+0.6
+0.3
stress
Gentamicin 0.008 mg/ml
+0.5
-0.3
+0.3
-0.4
-0.7
carbon source
2'-Deoxyinosine 5 mM (C)
+0.9
-0.0
-0.6
-0.5
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
-0.0
-1.4
-0.3
+0.6
seeds
Growth on radish seeds for 72 hours
-0.1
+0.4
-0.6
-0.6
+0.6
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
+0.3
-0.2
-0.7
+0.7
phage
P. simiae OR1 MOI 0.1
-1.0
+0.2
-0.4
+0.4
+0.5
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.8
+0.0
-0.3
-1.0
+0.2
supernatant control
Vogels_fungal_media 0.1X
+0.2
-0.0
+0.6
-1.3
+0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.6
+0.6
-0.1
-1.1
-0.1
agar plate interaction control
Taped volatile agar plate with no fungus
-0.3
-0.5
+1.1
-0.6
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.9
-0.4
+0.1
+0.4
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.7
-0.5
+0.6
+0.4
+0.6
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.7
+0.4
-0.7
-0.2
+0.3
stress
Nalidixic 0.025 mg/ml
+0.4
+0.2
-0.3
+0.9
-0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
+0.1
+1.1
-0.7
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.3
+0.5
-0.2
-0.2
+1.2
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
-0.4
-0.1
+0.8
+0.3
+0.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.8
-0.0
-0.7
+1.9
+0.9
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