Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13625 and PS417_13635 are separated by 164 nucleotidesPS417_13635 and PS417_13640 overlap by 8 nucleotidesPS417_13640 and PS417_13645 are separated by 30 nucleotidesPS417_13645 and PS417_13650 are separated by 22 nucleotides PS417_13625: PS417_13625 - cytochrome B561, at 2,999,595 to 3,000,137 _13625 PS417_13635: PS417_13635 - D-ribose transporter ATP-binding protein, at 3,000,302 to 3,001,834 _13635 PS417_13640: PS417_13640 - ribose ABC transporter permease, at 3,001,827 to 3,002,804 _13640 PS417_13645: PS417_13645 - ABC transporter, at 3,002,835 to 3,003,767 _13645 PS417_13650: PS417_13650 - LacI family transcriptional regulator, at 3,003,790 to 3,004,791 _13650
Group Condition PS417_13625 PS417_13635 PS417_13640 PS417_13645 PS417_13650
motility outer cut, LB soft agar motility assay -0.3 -0.2 -0.7 -0.6 -0.4
root root sample 6; outgrowth in LB -0.5 +0.0 -0.8 -1.2 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 -0.2 -0.9 -1.2 -0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.5 -0.3 -0.9 -0.7 -0.4
carbon source D-Fructose (C) +0.1 -0.9 -0.8 -0.7 +1.0
stress methylglyoxal 0.032 vol% +0.5 +0.1 -1.0 +0.2 -1.1
carbon source D-Fructose (C) +0.1 -0.6 -0.7 -1.1 +1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -0.1 -1.1 -0.7 +0.5
solid stress Fraxetin 3 mM; solid stress -0.3 -0.2 -0.7 +0.8 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.3 -0.6 +0.7 -0.2
seeds Growth on radish seeds for 72 hours -0.4 -0.2 -1.0 +0.2 +0.6
seeds Growth on radish seeds for 24 hours -0.3 +0.1 -1.4 +0.6 +0.3
stress Gentamicin 0.008 mg/ml +0.5 -0.3 +0.3 -0.4 -0.7
carbon source 2'-Deoxyinosine 5 mM (C) +0.9 -0.0 -0.6 -0.5 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 -0.0 -1.4 -0.3 +0.6
seeds Growth on radish seeds for 72 hours -0.1 +0.4 -0.6 -0.6 +0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.3 -0.2 -0.7 +0.7
phage P. simiae OR1 MOI 0.1 -1.0 +0.2 -0.4 +0.4 +0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.8 +0.0 -0.3 -1.0 +0.2
supernatant control Vogels_fungal_media 0.1X +0.2 -0.0 +0.6 -1.3 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.6 +0.6 -0.1 -1.1 -0.1
agar plate interaction control Taped volatile agar plate with no fungus -0.3 -0.5 +1.1 -0.6 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 -0.4 +0.1 +0.4 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 -0.5 +0.6 +0.4 +0.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.7 +0.4 -0.7 -0.2 +0.3
stress Nalidixic 0.025 mg/ml +0.4 +0.2 -0.3 +0.9 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 +0.1 +1.1 -0.7 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.3 +0.5 -0.2 -0.2 +1.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 -0.1 +0.8 +0.3 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -0.0 -0.7 +1.9 +0.9
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