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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13560 and PS417_13565 overlap by 4 nucleotides
PS417_13565 and PS417_13575 are separated by 585 nucleotides
PS417_13575 and PS417_13580 overlap by 39 nucleotides
PS417_13580 and PS417_13585 are separated by 143 nucleotides
PS417_13560: PS417_13560 - nickel permease, at 2,987,886 to 2,988,584
_13560
PS417_13565: PS417_13565 - permease, at 2,988,581 to 2,989,579
_13565
PS417_13575: PS417_13575 - succinylglutamate desuccinylase, at 2,990,165 to 2,991,163
_13575
PS417_13580: PS417_13580 - AraC family transcriptional regulator, at 2,991,125 to 2,991,982
_13580
PS417_13585: PS417_13585 - alpha/beta hydrolase, at 2,992,126 to 2,992,926
_13585
Group
Condition
PS417
_13560
PS417
_13565
PS417
_13575
PS417
_13580
PS417
_13585
root
root sample 6; outgrowth in LB
+0.1
-0.2
+0.1
-1.4
-0.2
motility
inner cut, LB soft agar motility assay
+0.3
-0.2
-0.5
-0.6
-0.4
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.1
+0.2
-0.8
-0.6
-0.2
soil
soil sample 8; outgrowth in LB
-0.0
+0.3
-1.1
-0.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.9
-0.0
+0.3
-0.6
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.2
-0.4
-0.7
+0.2
+0.3
phage
P. simiae ORA MOI 10
-0.5
-0.1
-0.3
+0.9
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
+0.1
+0.7
-0.7
+0.2
stress
methylglyoxal 0.032 vol%
+0.4
-0.7
+0.6
+0.1
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.5
-0.4
+0.7
+0.1
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.0
+0.8
-0.7
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.2
+0.5
-0.4
+0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.1
+0.3
-0.3
-0.5
-0.2
phage moi 100
Time6-phageFRS
+0.2
-0.0
+1.1
-0.6
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.4
-0.1
-0.5
+0.4
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.1
+0.8
-0.7
+0.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.4
+0.3
-0.3
+0.5
-0.3
phage
P. simiae Grant Run MOI 10
-0.4
+0.4
+0.6
+0.3
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.2
+0.5
+0.7
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.4
+0.4
+0.6
+0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.2
+1.0
-0.3
-0.0
seeds
Growth on radish seeds for 24 hours
+0.2
+0.2
+1.1
-0.3
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.2
+0.3
+0.7
+0.6
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+0.2
+0.5
+0.4
-0.2
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.3
+0.4
+1.2
+0.3
-0.7
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.2
-0.3
+1.1
+0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.1
+0.2
-0.1
+1.3
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.9
+0.3
+0.4
-0.1
+0.0
no phage control
Time6-nophage
-0.1
+0.0
+1.1
+0.3
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.1
+1.1
-0.1
+0.8
+0.2
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