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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13550 and PS417_13555 are separated by 2 nucleotides
PS417_13555 and PS417_13560 are separated by 7 nucleotides
PS417_13560 and PS417_13565 overlap by 4 nucleotides
PS417_13565 and PS417_13575 are separated by 585 nucleotides
PS417_13550: PS417_13550 - amino acid ABC transporter substrate-binding protein, at 2,986,317 to 2,987,171
_13550
PS417_13555: PS417_13555 - ABC transporter permease, at 2,987,174 to 2,987,878
_13555
PS417_13560: PS417_13560 - nickel permease, at 2,987,886 to 2,988,584
_13560
PS417_13565: PS417_13565 - permease, at 2,988,581 to 2,989,579
_13565
PS417_13575: PS417_13575 - succinylglutamate desuccinylase, at 2,990,165 to 2,991,163
_13575
Group
Condition
PS417
_13550
PS417
_13555
PS417
_13560
PS417
_13565
PS417
_13575
phage
JP1 MOI 1
+0.0
-1.4
-0.1
-0.2
-1.0
phage
P. simiae OR Antrim MOI 0.1
+0.2
-0.9
+0.1
-0.4
-0.8
phage
P. simiae ORA MOI 0.1
+0.3
-0.8
-0.2
+0.1
-1.1
phage
P. simiae Grant Run MOI 0.1
+0.4
-0.6
-0.3
-0.2
-0.8
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
-0.5
+0.2
+0.2
-1.0
carbon source
L-Valine (C)
+0.1
-1.0
+0.2
-0.2
-0.3
phage
P. simiae OR1 MOI 0.1
+0.4
-1.1
-0.1
-0.4
-0.0
motility
outer cut, LB soft agar motility assay
-0.3
-0.6
-0.0
-0.4
+0.5
soil
soil sample 6; outgrowth in LB
-0.1
-1.3
-0.1
+0.3
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-0.7
-0.3
+0.4
+0.2
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-0.4
+0.4
-0.2
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-0.4
+0.4
-0.4
+0.3
soil
soil sample 8; outgrowth in LB
+0.1
+0.5
-0.0
+0.3
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
+0.2
-0.5
-0.4
+0.7
agar plate interaction control
Taped volatile agar plate with no fungus
+0.4
+0.7
-0.0
-0.4
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.6
-0.1
+0.5
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.6
+0.5
-0.1
+0.0
-0.7
soil
soil sample 5; outgrowth in LB
+0.4
-0.5
+0.3
-0.2
+0.3
stress
methylglyoxal 0.032 vol%
-0.1
+0.3
+0.4
-0.7
+0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
+0.7
-0.1
-0.3
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.5
+0.0
+0.1
+1.1
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.2
+1.1
+0.3
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.4
+0.3
-0.1
+0.8
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
+0.4
-0.1
-0.4
+1.0
seeds
Growth on radish seeds for 24 hours
+0.2
-0.4
+0.2
+0.2
+1.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.3
-0.8
+0.3
+0.4
+1.2
no phage control
Time6-nophage
+0.1
+0.3
-0.1
+0.0
+1.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
+0.0
+0.9
+0.3
+0.4
stress
Neomycin 0.04 mg/ml
+0.4
+0.9
-0.0
-0.1
+0.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
-0.3
+0.1
+0.2
+1.0
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