Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13550 and PS417_13555 are separated by 2 nucleotidesPS417_13555 and PS417_13560 are separated by 7 nucleotidesPS417_13560 and PS417_13565 overlap by 4 nucleotidesPS417_13565 and PS417_13575 are separated by 585 nucleotides PS417_13550: PS417_13550 - amino acid ABC transporter substrate-binding protein, at 2,986,317 to 2,987,171 _13550 PS417_13555: PS417_13555 - ABC transporter permease, at 2,987,174 to 2,987,878 _13555 PS417_13560: PS417_13560 - nickel permease, at 2,987,886 to 2,988,584 _13560 PS417_13565: PS417_13565 - permease, at 2,988,581 to 2,989,579 _13565 PS417_13575: PS417_13575 - succinylglutamate desuccinylase, at 2,990,165 to 2,991,163 _13575
Group Condition PS417_13550 PS417_13555 PS417_13560 PS417_13565 PS417_13575
phage JP1 MOI 1 +0.0 -1.4 -0.1 -0.2 -1.0
phage P. simiae OR Antrim MOI 0.1 +0.2 -0.9 +0.1 -0.4 -0.8
phage P. simiae ORA MOI 0.1 +0.3 -0.8 -0.2 +0.1 -1.1
phage P. simiae Grant Run MOI 0.1 +0.4 -0.6 -0.3 -0.2 -0.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 -0.5 +0.2 +0.2 -1.0
carbon source L-Valine (C) +0.1 -1.0 +0.2 -0.2 -0.3
phage P. simiae OR1 MOI 0.1 +0.4 -1.1 -0.1 -0.4 -0.0
motility outer cut, LB soft agar motility assay -0.3 -0.6 -0.0 -0.4 +0.5
soil soil sample 6; outgrowth in LB -0.1 -1.3 -0.1 +0.3 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -0.7 -0.3 +0.4 +0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.4 +0.4 -0.2 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.4 +0.4 -0.4 +0.3
soil soil sample 8; outgrowth in LB +0.1 +0.5 -0.0 +0.3 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 +0.2 -0.5 -0.4 +0.7
agar plate interaction control Taped volatile agar plate with no fungus +0.4 +0.7 -0.0 -0.4 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.6 -0.1 +0.5 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 +0.5 -0.1 +0.0 -0.7
soil soil sample 5; outgrowth in LB +0.4 -0.5 +0.3 -0.2 +0.3
stress methylglyoxal 0.032 vol% -0.1 +0.3 +0.4 -0.7 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.7 -0.1 -0.3 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.5 +0.0 +0.1 +1.1 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.2 +1.1 +0.3 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.4 +0.3 -0.1 +0.8
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.4 -0.1 -0.4 +1.0
seeds Growth on radish seeds for 24 hours +0.2 -0.4 +0.2 +0.2 +1.1
rhizosphere rhizosphere sample 2; outgrowth in LB +0.3 -0.8 +0.3 +0.4 +1.2
no phage control Time6-nophage +0.1 +0.3 -0.1 +0.0 +1.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.0 +0.9 +0.3 +0.4
stress Neomycin 0.04 mg/ml +0.4 +0.9 -0.0 -0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.3 +0.1 +0.2 +1.0
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