Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01340 and PS417_01345 are separated by 233 nucleotidesPS417_01345 and PS417_01350 are separated by 38 nucleotidesPS417_01350 and PS417_01355 are separated by 411 nucleotidesPS417_01355 and PS417_01360 are separated by 202 nucleotides PS417_01340: PS417_01340 - agmatine deiminase, at 291,997 to 293,103 _01340 PS417_01345: PS417_01345 - hypothetical protein, at 293,337 to 293,555 _01345 PS417_01350: PS417_01350 - TonB-dependent receptor, at 293,594 to 295,681 _01350 PS417_01355: PS417_01355 - membrane protein, at 296,093 to 297,409 _01355 PS417_01360: PS417_01360 - 4-oxalocrotonate tautomerase, at 297,612 to 297,815 _01360
Group Condition PS417_01340 PS417_01345 PS417_01350 PS417_01355 PS417_01360
soil soil sample 7; outgrowth in LB -2.3 N.D. -0.3 -0.4 -0.6
soil soil sample 2; outgrowth in LB -2.0 N.D. -0.1 -0.4 -0.7
soil soil sample 3; outgrowth in LB -1.8 N.D. -0.1 -0.5 -0.5
soil soil sample 4; outgrowth in LB -1.6 N.D. +0.2 -0.3 -0.5
root root sample 6; outgrowth in LB -0.8 N.D. -0.3 -0.4 -0.5
agar plate interaction control Taped volatile agar plate with no fungus -0.8 N.D. -0.2 -0.3 -0.6
soil soil sample 5; outgrowth in LB -1.2 N.D. +0.0 -0.5 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 N.D. -0.1 -0.0 -1.0
rhizosphere rhizosphere sample 2; outgrowth in LB -0.9 N.D. -0.0 -0.4 -0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.9 N.D. +0.0 -0.2 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 N.D. -0.2 +0.0 -0.6
phage P. simiae OR Antrim MOI 10 -1.1 N.D. +0.4 -0.2 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 N.D. +0.1 -0.2 -0.7
stress methylglyoxal 0.032 vol% -0.6 N.D. +0.1 -0.3 -0.6
phage P. simiae Grant Run MOI 10 -0.6 N.D. -0.2 +0.2 -0.8
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.8 N.D. +0.2 -0.3 -0.3
seeds Growth on radish seeds for 72 hours -1.2 N.D. -0.1 +0.6 -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.1 N.D. -0.2 -0.1 +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.3 N.D. +0.1 -0.2 +0.4
solid stress Fraxetin 3 mM; solid stress -0.8 N.D. +0.1 +0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 N.D. +0.3 +0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 N.D. +0.3 -0.3 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 N.D. +0.2 -0.0 +0.7
carbon source L-Valine (C) +0.6 N.D. +0.2 +0.3 -1.0
stress Gentamicin 0.008 mg/ml +1.2 N.D. +0.4 +0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 N.D. +0.3 +0.0 +2.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 N.D. +0.2 +0.1 +3.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.8 N.D. +0.1 +4.6 +0.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.1 N.D. +0.1 +7.5 -0.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +2.4 N.D. +0.1 +7.6 -0.2
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