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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_01330 and PS417_01335 are separated by 151 nucleotides
PS417_01335 and PS417_01340 are separated by 121 nucleotides
PS417_01340 and PS417_01345 are separated by 233 nucleotides
PS417_01345 and PS417_01350 are separated by 38 nucleotides
PS417_01330: PS417_01330 - dTDP-glucose 4,6-dehydratase, at 287,741 to 288,823
_01330
PS417_01335: PS417_01335 - hypothetical protein, at 288,975 to 291,875
_01335
PS417_01340: PS417_01340 - agmatine deiminase, at 291,997 to 293,103
_01340
PS417_01345: PS417_01345 - hypothetical protein, at 293,337 to 293,555
_01345
PS417_01350: PS417_01350 - TonB-dependent receptor, at 293,594 to 295,681
_01350
Group
Condition
PS417
_01330
PS417
_01335
PS417
_01340
PS417
_01345
PS417
_01350
soil
soil sample 7; outgrowth in LB
N.D.
-0.3
-2.3
N.D.
-0.3
soil
soil sample 2; outgrowth in LB
N.D.
+0.0
-2.0
N.D.
-0.1
soil
soil sample 3; outgrowth in LB
N.D.
+0.0
-1.8
N.D.
-0.1
soil
soil sample 4; outgrowth in LB
N.D.
+0.0
-1.6
N.D.
+0.2
root
root sample 6; outgrowth in LB
N.D.
-0.3
-0.8
N.D.
-0.3
seeds
Growth on radish seeds for 72 hours
N.D.
-0.0
-1.2
N.D.
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-0.4
-0.7
N.D.
-0.1
soil
soil sample 5; outgrowth in LB
N.D.
-0.1
-1.2
N.D.
+0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
-0.3
-0.8
N.D.
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.0
-1.3
N.D.
+0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-0.3
-0.9
N.D.
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.1
-0.8
N.D.
-0.2
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-0.1
-0.8
N.D.
-0.2
carbon source
Carbon source L-Citrulline 10 mM
N.D.
-0.3
-0.6
N.D.
-0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.3
-1.1
N.D.
-0.2
motility
inner cut, LB soft agar motility assay
N.D.
-0.1
-1.0
N.D.
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
N.D.
+0.1
-1.1
N.D.
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.2
-0.9
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.3
-1.1
N.D.
-0.1
solid stress
Fraxetin 3 mM; solid stress
N.D.
-0.1
-0.8
N.D.
+0.2
phage
P. simiae OR Antrim MOI 10
N.D.
+0.2
-1.1
N.D.
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.2
-0.9
N.D.
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
+0.1
-0.8
N.D.
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.3
-0.8
N.D.
+0.2
seeds
Growth on radish seeds for 24 hours
N.D.
+0.4
-0.8
N.D.
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.4
-0.9
N.D.
+0.2
carbon source
L-Valine (C)
N.D.
-0.4
+0.6
N.D.
+0.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
+0.2
+1.1
N.D.
+0.1
stress
Gentamicin 0.008 mg/ml
N.D.
+0.3
+1.2
N.D.
+0.4
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
+0.3
+2.4
N.D.
+0.1
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