Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01330 and PS417_01335 are separated by 151 nucleotidesPS417_01335 and PS417_01340 are separated by 121 nucleotidesPS417_01340 and PS417_01345 are separated by 233 nucleotidesPS417_01345 and PS417_01350 are separated by 38 nucleotides PS417_01330: PS417_01330 - dTDP-glucose 4,6-dehydratase, at 287,741 to 288,823 _01330 PS417_01335: PS417_01335 - hypothetical protein, at 288,975 to 291,875 _01335 PS417_01340: PS417_01340 - agmatine deiminase, at 291,997 to 293,103 _01340 PS417_01345: PS417_01345 - hypothetical protein, at 293,337 to 293,555 _01345 PS417_01350: PS417_01350 - TonB-dependent receptor, at 293,594 to 295,681 _01350
Group Condition PS417_01330 PS417_01335 PS417_01340 PS417_01345 PS417_01350
soil soil sample 7; outgrowth in LB N.D. -0.3 -2.3 N.D. -0.3
soil soil sample 2; outgrowth in LB N.D. +0.0 -2.0 N.D. -0.1
soil soil sample 3; outgrowth in LB N.D. +0.0 -1.8 N.D. -0.1
soil soil sample 4; outgrowth in LB N.D. +0.0 -1.6 N.D. +0.2
root root sample 6; outgrowth in LB N.D. -0.3 -0.8 N.D. -0.3
seeds Growth on radish seeds for 72 hours N.D. -0.0 -1.2 N.D. -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 -0.7 N.D. -0.1
soil soil sample 5; outgrowth in LB N.D. -0.1 -1.2 N.D. +0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.3 -0.8 N.D. -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.0 -1.3 N.D. +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.3 -0.9 N.D. +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.1 -0.8 N.D. -0.2
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.1 -0.8 N.D. -0.2
carbon source Carbon source L-Citrulline 10 mM N.D. -0.3 -0.6 N.D. -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.3 -1.1 N.D. -0.2
motility inner cut, LB soft agar motility assay N.D. -0.1 -1.0 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.1 -1.1 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.9 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -1.1 N.D. -0.1
solid stress Fraxetin 3 mM; solid stress N.D. -0.1 -0.8 N.D. +0.2
phage P. simiae OR Antrim MOI 10 N.D. +0.2 -1.1 N.D. +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 -0.9 N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.1 -0.8 N.D. +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.3 -0.8 N.D. +0.2
seeds Growth on radish seeds for 24 hours N.D. +0.4 -0.8 N.D. +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 -0.9 N.D. +0.2
carbon source L-Valine (C) N.D. -0.4 +0.6 N.D. +0.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +0.2 +1.1 N.D. +0.1
stress Gentamicin 0.008 mg/ml N.D. +0.3 +1.2 N.D. +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +0.3 +2.4 N.D. +0.1
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