Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13230 and PS417_13235 overlap by 4 nucleotidesPS417_13235 and PS417_13240 overlap by 4 nucleotidesPS417_13240 and PS417_13245 overlap by 4 nucleotidesPS417_13245 and PS417_13250 overlap by 7 nucleotides PS417_13230: PS417_13230 - cytochrome P450, at 2,892,869 to 2,894,047 _13230 PS417_13235: PS417_13235 - siderophore biosynthesis protein, at 2,894,044 to 2,895,714 _13235 PS417_13240: PS417_13240 - siderophore biosynthesis protein, at 2,895,711 to 2,896,745 _13240 PS417_13245: PS417_13245 - saccharopine dehydrogenase, at 2,896,742 to 2,897,845 _13245 PS417_13250: PS417_13250 - ABC transporter, at 2,897,839 to 2,899,584 _13250
Group Condition PS417_13230 PS417_13235 PS417_13240 PS417_13245 PS417_13250
rhizosphere rhizosphere sample 8; outgrowth in LB N.D. -0.3 -0.5 -1.5 -0.3
phage JP1 MOI 10 N.D. -0.4 -0.5 -1.0 -0.5
seeds Growth on radish seeds for 72 hours N.D. -0.4 -0.1 -0.6 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.1 -0.3 +0.2 -1.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.3 -0.1 +0.2 -1.3
phage JP1 MOI 1 N.D. +0.1 +0.2 -1.0 -0.8
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.7 +0.4 -0.6 -0.4
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.3 -0.9 +0.7 -0.7
phage Wcs_0.1 N.D. +0.3 -0.7 +0.2 -0.7
seeds Growth on radish seeds for 72 hours N.D. +0.4 -0.6 +0.3 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 -0.4 -0.9 +0.5
stress methylglyoxal 0.032 vol% N.D. -1.1 -1.0 +0.4 +0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.8 -0.2 -0.6 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.1 +0.5 +0.6 -1.9
soil soil sample 5; outgrowth in LB N.D. -0.5 -0.4 -1.0 +1.1
soil soil sample 6; outgrowth in LB N.D. +0.1 -0.7 -0.5 +0.5
carbon source 2-Deoxy-D-ribonic lithium salt 10 mM (C) N.D. -0.3 +0.2 -0.8 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -0.7 +0.6 +0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.2 +0.1 -0.9 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 +0.3 +0.7 +0.3
phage Wcs_1 N.D. -0.4 +0.6 +0.6 -0.4
agar plate interaction control Taped volatile agar plate with no fungus N.D. +0.1 -0.7 +0.4 +1.0
phage P. simiae ORA MOI 10 N.D. -0.4 +0.5 +0.9 -0.2
carbon source L-Valine (C) N.D. +0.1 -0.2 +1.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.4 +1.7 +0.1 -0.2
phage P. simiae OR Antrim MOI 1 N.D. +0.4 +0.2 +0.9 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.5 -0.6 +0.8 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 +1.0 -1.0 +0.9
seeds Growth on radish seeds for 72 hours N.D. +1.1 +0.2 +0.6 -0.6
soil soil sample 2; outgrowth in LB N.D. -0.0 +0.9 +0.7 +0.5
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