Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13205 and PS417_13210 overlap by 4 nucleotidesPS417_13210 and PS417_13215 overlap by 4 nucleotidesPS417_13215 and PS417_13220 overlap by 8 nucleotidesPS417_13220 and PS417_13225 overlap by 4 nucleotides PS417_13205: PS417_13205 - isochorismate-pyruvate lyase, at 2,888,360 to 2,888,668 _13205 PS417_13210: PS417_13210 - enantio-pyochelin biosynthetic protein PchC, at 2,888,665 to 2,889,423 _13210 PS417_13215: PS417_13215 - Triostin synthetase I, at 2,889,420 to 2,891,018 _13215 PS417_13220: PS417_13220 - hypothetical protein, at 2,891,011 to 2,891,730 _13220 PS417_13225: PS417_13225 - thiazolinyl imide reductase, at 2,891,727 to 2,892,872 _13225
Group Condition PS417_13205 PS417_13210 PS417_13215 PS417_13220 PS417_13225
seeds Growth on radish seeds for 72 hours -1.5 N.D. -0.6 N.D. N.D.
phage JP1 MOI 1 -1.4 N.D. -0.6 N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -1.6 N.D. -0.3 N.D. N.D.
solid stress Scopoletin 3 mM; solid stress -1.6 N.D. -0.3 N.D. N.D.
solid stress Scopoletin 0.5 mM; solid stress -1.4 N.D. -0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.1 N.D. -0.4 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.7 N.D. +0.3 N.D. N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 N.D. -0.7 N.D. N.D.
supernatant control Vogels_fungal_media 0.1X -1.5 N.D. +0.2 N.D. N.D.
no phage control no phage control -1.4 N.D. +0.2 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.4 N.D. +0.1 N.D. N.D.
liquid stress Fraxetin 3 mM; liquid stress -1.4 N.D. +0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.5 N.D. +0.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.2 N.D. +0.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 N.D. +0.3 N.D. N.D.
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -1.3 N.D. +0.5 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 N.D. +0.3 N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -1.1 N.D. +0.3 N.D. N.D.
carbon source Carbon source D-Mannitol 10 mM -1.1 N.D. +0.4 N.D. N.D.
soil soil sample 5; outgrowth in LB +0.6 N.D. -0.8 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 N.D. +0.8 N.D. N.D.
stress 1-ethyl-3-methylimidazolium chloride 200 mM +1.0 N.D. -0.6 N.D. N.D.
stress Gentamicin 0.008 mg/ml +1.2 N.D. -0.7 N.D. N.D.
stress methylglyoxal 0.032 vol% +1.3 N.D. -0.3 N.D. N.D.
stress Chloramphenicol 0.015 mg/ml +1.3 N.D. -0.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 N.D. -0.2 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.4 N.D. -0.1 N.D. N.D.
phage moi 100 Time6-phageFRS +1.3 N.D. +0.1 N.D. N.D.
stress Nalidixic 0.015 mg/ml +1.3 N.D. +0.2 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.8 N.D. +0.1 N.D. N.D.
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