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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13135 and PS417_13140 are separated by 12 nucleotides
PS417_13140 and PS417_13145 are separated by 32 nucleotides
PS417_13145 and PS417_13150 are separated by 53 nucleotides
PS417_13150 and PS417_13155 are separated by 125 nucleotides
PS417_13135: PS417_13135 - hypothetical protein, at 2,871,037 to 2,871,351
_13135
PS417_13140: PS417_13140 - oxidoreductase, at 2,871,364 to 2,872,521
_13140
PS417_13145: PS417_13145 - 50S ribosomal protein L21, at 2,872,554 to 2,873,033
_13145
PS417_13150: PS417_13150 - TetR family transcriptional regulator, at 2,873,087 to 2,873,650
_13150
PS417_13155: PS417_13155 - phosphatidylserine synthase, at 2,873,776 to 2,875,119
_13155
Group
Condition
PS417
_13135
PS417
_13140
PS417
_13145
PS417
_13150
PS417
_13155
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
-3.0
-1.1
N.D.
+0.1
soil
soil sample 5; outgrowth in LB
+0.4
-4.7
+0.5
N.D.
-0.2
seeds
Growth on radish seeds for 72 hours
+0.5
-2.3
-0.4
N.D.
-0.5
stress
Doxycycline hyclate 0.001 mg/ml
+0.2
-1.5
-1.2
N.D.
+0.0
seeds
Growth on radish seeds for 72 hours
-0.1
-1.4
-0.8
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
-3.4
-0.3
N.D.
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
-2.4
-0.5
N.D.
+0.3
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+0.4
-1.6
-0.8
N.D.
-0.1
phage
P. simiae Grant Run MOI 0.1
-0.2
-1.3
-0.5
N.D.
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.1
-1.1
-0.9
N.D.
-0.2
phage
P. simiae Grant Run MOI 0.1
-0.1
-2.0
+0.3
N.D.
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.1
-2.1
-0.1
N.D.
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
+0.1
-2.5
+0.6
N.D.
-0.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.4
-1.6
-1.0
N.D.
+0.2
seeds
Growth on radish seeds for 24 hours
+0.4
-1.1
-1.0
N.D.
-0.1
root
root sample 6; outgrowth in LB
-0.5
-0.4
-1.0
N.D.
+0.3
stress
1-ethyl-3-methylimidazolium acetate 35 mM
+0.2
-0.7
-1.1
N.D.
+0.1
phage
JP1 MOI 10
-1.0
+0.5
-0.7
N.D.
-0.1
carbon source
2'-Deoxyinosine 5 mM (C)
-0.3
+1.0
-1.1
N.D.
-0.4
phage
JP1 MOI 1
-0.1
+1.2
-1.2
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.9
-1.1
-0.4
N.D.
+0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.1
-0.7
+1.0
N.D.
-0.6
phage
P. simiae OR Antrim MOI 0.1
-0.2
-0.9
+1.0
N.D.
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.9
-1.4
+0.7
N.D.
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.9
-0.7
N.D.
-0.2
nitrogen source
Ammonium chloride (N)
-0.1
+1.3
-0.6
N.D.
+0.2
nophagecontrol
Only library
-0.3
+0.9
+1.2
N.D.
-0.4
agar plate interaction control
Taped volatile agar plate with no fungus
-0.1
+1.1
+0.8
N.D.
-0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.7
+1.2
+0.5
N.D.
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
+3.5
+0.9
N.D.
+0.1
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