Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13130 and PS417_13135 are separated by 184 nucleotidesPS417_13135 and PS417_13140 are separated by 12 nucleotidesPS417_13140 and PS417_13145 are separated by 32 nucleotidesPS417_13145 and PS417_13150 are separated by 53 nucleotides PS417_13130: PS417_13130 - DNA repair protein, at 2,870,019 to 2,870,852 _13130 PS417_13135: PS417_13135 - hypothetical protein, at 2,871,037 to 2,871,351 _13135 PS417_13140: PS417_13140 - oxidoreductase, at 2,871,364 to 2,872,521 _13140 PS417_13145: PS417_13145 - 50S ribosomal protein L21, at 2,872,554 to 2,873,033 _13145 PS417_13150: PS417_13150 - TetR family transcriptional regulator, at 2,873,087 to 2,873,650 _13150
Group Condition PS417_13130 PS417_13135 PS417_13140 PS417_13145 PS417_13150
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 -3.0 -1.1 N.D.
soil soil sample 5; outgrowth in LB N.D. +0.4 -4.7 +0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.8 -3.4 -0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.2 -2.4 -0.5 N.D.
stress Doxycycline hyclate 0.001 mg/ml N.D. +0.2 -1.5 -1.2 N.D.
seeds Growth on radish seeds for 72 hours N.D. -0.1 -1.4 -0.8 N.D.
seeds Growth on radish seeds for 72 hours N.D. +0.5 -2.3 -0.4 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.4 -1.6 -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.1 -2.1 -0.1 N.D.
stress 1-ethyl-3-methylimidazolium chloride 200 mM N.D. +0.4 -1.6 -0.8 N.D.
phage P. simiae Grant Run MOI 0.1 N.D. -0.2 -1.3 -0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.1 -1.1 -0.9 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. +0.1 -2.5 +0.6 N.D.
phage P. simiae Grant Run MOI 0.1 N.D. -0.1 -2.0 +0.3 N.D.
seeds Growth on radish seeds for 24 hours N.D. +0.4 -1.1 -1.0 N.D.
stress 1-ethyl-3-methylimidazolium acetate 35 mM N.D. +0.2 -0.7 -1.1 N.D.
rhizosphere rhizosphere sample 8; outgrowth in LB N.D. -0.1 -1.6 +0.2 N.D.
phage JP1 MOI 10 N.D. -1.0 +0.5 -0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.9 -1.1 -0.4 N.D.
carbon source 2'-Deoxyinosine 5 mM (C) N.D. -0.3 +1.0 -1.1 N.D.
phage P. simiae OR Antrim MOI 0.1 N.D. -0.2 -0.9 +1.0 N.D.
phage JP1 MOI 1 N.D. -0.1 +1.2 -1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.9 -1.4 +0.7 N.D.
nitrogen source Ammonium chloride (N) N.D. -0.1 +1.3 -0.6 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 +0.9 -0.7 N.D.
soil soil sample 8; outgrowth in LB N.D. +0.4 +1.0 -0.5 N.D.
nophagecontrol Only library N.D. -0.3 +0.9 +1.2 N.D.
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.1 +1.1 +0.8 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.7 +1.2 +0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.4 +3.5 +0.9 N.D.
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