Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13125 and PS417_13130 are separated by 3 nucleotidesPS417_13130 and PS417_13135 are separated by 184 nucleotidesPS417_13135 and PS417_13140 are separated by 12 nucleotidesPS417_13140 and PS417_13145 are separated by 32 nucleotides PS417_13125: PS417_13125 - ATP-dependent DNA ligase, at 2,867,556 to 2,870,015 _13125 PS417_13130: PS417_13130 - DNA repair protein, at 2,870,019 to 2,870,852 _13130 PS417_13135: PS417_13135 - hypothetical protein, at 2,871,037 to 2,871,351 _13135 PS417_13140: PS417_13140 - oxidoreductase, at 2,871,364 to 2,872,521 _13140 PS417_13145: PS417_13145 - 50S ribosomal protein L21, at 2,872,554 to 2,873,033 _13145
Group Condition PS417_13125 PS417_13130 PS417_13135 PS417_13140 PS417_13145
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 N.D. -0.4 -3.0 -1.1
soil soil sample 5; outgrowth in LB +0.1 N.D. +0.4 -4.7 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 N.D. +0.8 -3.4 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.0 N.D. +0.2 -2.4 -0.5
stress Doxycycline hyclate 0.001 mg/ml -0.0 N.D. +0.2 -1.5 -1.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 N.D. +0.4 -1.6 -1.0
seeds Growth on radish seeds for 72 hours -0.1 N.D. +0.5 -2.3 -0.4
seeds Growth on radish seeds for 72 hours -0.0 N.D. -0.1 -1.4 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 N.D. +0.1 -1.1 -0.9
stress 1-ethyl-3-methylimidazolium chloride 200 mM -0.1 N.D. +0.4 -1.6 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 N.D. +0.1 -2.1 -0.1
stress 1-ethyl-3-methylimidazolium acetate 35 mM -0.4 N.D. +0.2 -0.7 -1.1
seeds Growth on radish seeds for 24 hours -0.1 N.D. +0.4 -1.1 -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.2 N.D. +0.1 -2.5 +0.6
root root sample 6; outgrowth in LB +0.4 N.D. -0.5 -0.4 -1.0
phage P. simiae Grant Run MOI 0.1 +0.5 N.D. -0.1 -2.0 +0.3
rhizosphere rhizosphere sample 8; outgrowth in LB +0.3 N.D. -0.1 -1.6 +0.2
phage JP1 MOI 10 +0.5 N.D. -1.0 +0.5 -0.7
carbon source 2'-Deoxyinosine 5 mM (C) -0.2 N.D. -0.3 +1.0 -1.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 N.D. -0.2 +0.9 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 N.D. +0.9 -1.1 -0.4
phage P. simiae OR Antrim MOI 0.1 -0.1 N.D. -0.2 -0.9 +1.0
phage JP1 MOI 1 +0.1 N.D. -0.1 +1.2 -1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 N.D. +0.5 +0.9 -0.7
phage P. simiae OR Antrim MOI 10 -0.5 N.D. +0.1 +1.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 N.D. +0.9 -1.4 +0.7
agar plate interaction control Taped volatile agar plate with no fungus -0.2 N.D. -0.1 +1.1 +0.8
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.6 N.D. +0.7 +1.2 +0.5
nophagecontrol Only library +0.4 N.D. -0.3 +0.9 +1.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. +0.4 +3.5 +0.9
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