Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13075 and PS417_13080 overlap by 4 nucleotidesPS417_13080 and PS417_13085 are separated by 125 nucleotidesPS417_13085 and PS417_13090 are separated by 228 nucleotidesPS417_13090 and PS417_13095 overlap by 7 nucleotides PS417_13075: PS417_13075 - hypothetical protein, at 2,857,276 to 2,857,632 _13075 PS417_13080: PS417_13080 - LysR family transcriptional regulator, at 2,857,629 to 2,858,537 _13080 PS417_13085: PS417_13085 - FMN-dependent NADH-azoreductase, at 2,858,663 to 2,859,274 _13085 PS417_13090: PS417_13090 - dienelactone hydrolase, at 2,859,503 to 2,860,540 _13090 PS417_13095: PS417_13095 - MFS transporter, at 2,860,534 to 2,861,709 _13095
Group Condition PS417_13075 PS417_13080 PS417_13085 PS417_13090 PS417_13095
soil soil sample 6; outgrowth in LB -1.7 N.D. -0.7 -1.3 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -3.4 N.D. -0.2 -0.6 +0.4
rhizosphere rhizosphere sample 2; outgrowth in LB -1.5 N.D. -0.5 -1.1 -0.1
phage P. simiae ORA MOI 0.1 -2.2 N.D. -0.5 -0.2 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.7 N.D. +0.1 -0.3 -0.0
phage P. simiae ORA MOI 0.1 -2.9 N.D. +0.2 -0.3 +0.2
soil soil sample 4; outgrowth in LB -1.2 N.D. -1.5 +0.2 -0.3
carbon source D-Glucose (C) -1.5 N.D. -0.6 -0.3 -0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.4 N.D. +0.1 -0.1 -0.1
no phage control Time6-nophage -2.0 N.D. -0.5 +0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -2.2 N.D. -0.1 +0.1 -0.1
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -2.9 N.D. +0.1 +0.6 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.8 N.D. +0.4 +0.9 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.5 N.D. -0.1 +0.8 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 N.D. -0.4 -0.7 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -1.7 N.D. +0.4 +0.2 +0.0
solid stress Fraxetin 3 mM; solid stress -0.6 N.D. -1.0 +0.6 +0.2
phage JP1 MOI 10 -1.8 N.D. +0.2 +1.3 -0.4
carbon source Carbon source D-Mannitol 5 mM -1.3 N.D. -0.3 +0.8 +0.3
stress Nalidixic 0.025 mg/ml -1.0 N.D. -0.4 +1.0 +0.2
seeds Growth on radish seeds for 24 hours +1.2 N.D. -0.4 -0.2 -0.8
phage P. simiae Grant Run MOI 0.1 -1.4 N.D. +0.1 +0.8 +0.5
seeds Growth on radish seeds for 72 hours +0.7 N.D. +0.8 -0.8 -0.2
carbon source 2'-Deoxyinosine 5 mM (C) +0.8 N.D. +0.7 -0.9 +0.2
agar plate interaction control Taped volatile agar plate with no fungus -0.5 N.D. +0.8 +0.9 -0.2
carbon source L-Valine (C) -0.7 N.D. +0.3 +0.7 +0.8
seeds Growth on radish seeds for 72 hours +0.9 N.D. -0.4 +1.5 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 N.D. +0.5 +0.6 +0.5
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +2.8 N.D. +0.1 -0.5 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.0 N.D. +1.9 +0.4 -0.0
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