Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13065 and PS417_13070 overlap by 4 nucleotidesPS417_13070 and PS417_13075 are separated by 385 nucleotidesPS417_13075 and PS417_13080 overlap by 4 nucleotidesPS417_13080 and PS417_13085 are separated by 125 nucleotides PS417_13065: PS417_13065 - UDP pyrophosphate phosphatase, at 2,855,680 to 2,856,510 _13065 PS417_13070: PS417_13070 - membrane protein, at 2,856,507 to 2,856,890 _13070 PS417_13075: PS417_13075 - hypothetical protein, at 2,857,276 to 2,857,632 _13075 PS417_13080: PS417_13080 - LysR family transcriptional regulator, at 2,857,629 to 2,858,537 _13080 PS417_13085: PS417_13085 - FMN-dependent NADH-azoreductase, at 2,858,663 to 2,859,274 _13085
Group Condition PS417_13065 PS417_13070 PS417_13075 PS417_13080 PS417_13085
stress R2A with Polymyxin B sulfate 0.003 mg/ml -3.2 N.D. -0.7 N.D. -0.2
phage P. simiae ORA MOI 0.1 -0.8 N.D. -2.9 N.D. +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 N.D. -3.4 N.D. -0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.5 N.D. -1.0 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -2.3 N.D. -0.4 N.D. -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.4 N.D. -0.5 N.D. -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 N.D. -2.8 N.D. +0.4
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.2 N.D. -2.9 N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. -2.5 N.D. -0.1
soil soil sample 4; outgrowth in LB -0.2 N.D. -1.2 N.D. -1.5
phage P. simiae ORA MOI 0.1 +0.2 N.D. -2.2 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.1 N.D. -1.0 N.D. -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 N.D. -2.7 N.D. +0.1
soil soil sample 6; outgrowth in LB +0.0 N.D. -1.7 N.D. -0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 N.D. -2.4 N.D. +0.1
phage P. simiae Grant Run MOI 0.1 -1.1 N.D. -1.4 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 N.D. -0.8 N.D. -0.8
phage P. simiae Grant Run MOI 0.1 -0.9 N.D. -1.4 N.D. +0.1
phage JP1 MOI 10 -0.9 N.D. -1.0 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. -2.2 N.D. -0.1
no phage control Time6-nophage +0.3 N.D. -2.0 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.3 N.D. +0.2 N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.6 N.D. -1.7 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 N.D. -1.3 N.D. +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. -1.5 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.5 N.D. -0.6 N.D. +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.4 N.D. -1.4 N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.1 N.D. +0.9 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.0 N.D. +2.8 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 N.D. +1.0 N.D. +1.9
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