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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_13020 and PS417_13025 are separated by 151 nucleotides
PS417_13025 and PS417_13030 are separated by 173 nucleotides
PS417_13030 and PS417_13035 are separated by 5 nucleotides
PS417_13035 and PS417_13040 are separated by 41 nucleotides
PS417_13020: PS417_13020 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 2,844,191 to 2,845,243
_13020
PS417_13025: PS417_13025 - MarR family transcriptional regulator, at 2,845,395 to 2,845,829
_13025
PS417_13030: PS417_13030 - (2Fe-2S)-binding protein, at 2,846,003 to 2,846,485
_13030
PS417_13035: PS417_13035 - aldehyde dehydrogenase, at 2,846,491 to 2,850,018
_13035
PS417_13040: PS417_13040 - TetR family transcriptional regulator, at 2,850,060 to 2,850,707
_13040
Group
Condition
PS417
_13020
PS417
_13025
PS417
_13030
PS417
_13035
PS417
_13040
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.6
N.D.
N.D.
-0.1
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.7
N.D.
N.D.
-0.0
-0.2
soil
soil sample 2; outgrowth in LB
-0.6
N.D.
N.D.
-0.1
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.5
N.D.
N.D.
-0.1
-0.2
phage
P. simiae Grant Run MOI 10
-0.5
N.D.
N.D.
-0.0
-0.3
phage
JP1 MOI 1
-0.4
N.D.
N.D.
+0.1
-0.4
phage
Wcs_0.1
-0.5
N.D.
N.D.
+0.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
N.D.
N.D.
+0.2
-0.4
motility
outer cut, LB soft agar motility assay
-0.6
N.D.
N.D.
-0.1
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
N.D.
N.D.
+0.2
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
N.D.
N.D.
+0.0
+0.2
solid stress
Fraxetin 3 mM; solid stress
-0.6
N.D.
N.D.
-0.1
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
N.D.
N.D.
+0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
N.D.
N.D.
+0.3
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-0.5
N.D.
N.D.
+0.0
+0.3
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.5
N.D.
N.D.
-0.0
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.5
N.D.
N.D.
+0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
N.D.
N.D.
+0.3
+0.2
stress
TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
+0.4
N.D.
N.D.
-0.1
-0.2
phage
P. simiae OR1 MOI 0.1
+0.6
N.D.
N.D.
-0.0
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
N.D.
N.D.
+0.1
-0.3
phage
JP1 MOI 0.1
+0.4
N.D.
N.D.
+0.1
-0.3
phage
P. simiae Grant Run MOI 0.1
+0.5
N.D.
N.D.
+0.1
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.8
N.D.
N.D.
-0.0
-0.2
seeds
Growth on radish seeds for 24 hours
+0.4
N.D.
N.D.
-0.1
+0.4
phage
JP1 MOI 10
+0.7
N.D.
N.D.
+0.2
-0.0
phage
P. simiae OR Antrim MOI 1
+0.7
N.D.
N.D.
+0.2
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.7
N.D.
N.D.
+0.2
+0.0
agar plate interaction control
Taped volatile agar plate with no fungus
+0.4
N.D.
N.D.
+0.0
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.0
N.D.
N.D.
+0.0
+0.9
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