Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_13005 and PS417_13010 are separated by 14 nucleotidesPS417_13010 and PS417_13015 overlap by 4 nucleotidesPS417_13015 and PS417_13020 are separated by 308 nucleotidesPS417_13020 and PS417_13025 are separated by 151 nucleotides PS417_13005: PS417_13005 - Asp/Glu racemase, at 2,841,188 to 2,841,940 _13005 PS417_13010: PS417_13010 - alpha/beta hydrolase, at 2,841,955 to 2,842,752 _13010 PS417_13015: PS417_13015 - 6-hydroxynicotinate 3-monooxygenase, at 2,842,749 to 2,843,882 _13015 PS417_13020: PS417_13020 - 2,5-dihydroxypyridine 5,6-dioxygenase, at 2,844,191 to 2,845,243 _13020 PS417_13025: PS417_13025 - MarR family transcriptional regulator, at 2,845,395 to 2,845,829 _13025
Group Condition PS417_13005 PS417_13010 PS417_13015 PS417_13020 PS417_13025
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -6.2 +0.1 -0.7 N.D.
phage JP1 MOI 1 -0.5 -1.5 -0.3 -0.4 N.D.
solid stress Fraxetin 3 mM; solid stress -0.2 -1.3 -0.2 -0.6 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -1.5 +0.2 -0.6 N.D.
soil soil sample 6; outgrowth in LB +0.0 -1.7 -0.1 -0.3 N.D.
phage Wcs_0.1 +0.1 -1.8 +0.1 -0.5 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.3 -1.3 -0.3 -0.0 N.D.
motility inner cut, LB soft agar motility assay -0.6 -0.9 -0.1 -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 -2.5 -0.0 +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -1.4 -0.2 +0.0 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB -0.3 -1.4 +0.1 -0.1 N.D.
carbon source L-Valine (C) -0.6 -1.0 -0.1 +0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 -0.9 -0.3 +0.4 N.D.
stress L-Lysine 60 mM -0.3 -1.4 +0.2 +0.0 N.D.
root root sample 6; outgrowth in LB +0.4 -1.7 -0.1 -0.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 -1.5 +0.2 -0.2 N.D.
phage P. simiae OR Antrim MOI 10 -0.5 -1.0 +0.2 +0.2 N.D.
phage JP1 MOI 0.1 -0.1 -1.5 +0.0 +0.4 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.9 -2.3 +0.4 -0.0 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x -0.1 -1.3 +0.4 +0.0 N.D.
nophagecontrol Only library +0.2 -0.9 -0.5 +0.3 N.D.
phage P. simiae Grant Run MOI 0.1 +0.4 -1.3 -0.0 +0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.6 -0.3 +0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 +1.0 -0.5 -0.2 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 +0.3 +0.5 +0.4 N.D.
phage P. simiae OR1 MOI 0.1 +0.6 -0.7 +0.3 +0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.3 +1.2 +0.4 -0.3 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.3 +1.0 -0.3 +0.2 N.D.
nitrogen source Ammonium chloride (N) +0.3 +1.4 +0.0 -0.1 N.D.
seeds Growth on radish seeds for 72 hours +0.4 +1.5 -0.2 +0.5 N.D.
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