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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_01290 and PS417_01295 overlap by 4 nucleotides
PS417_01295 and PS417_01300 are separated by 126 nucleotides
PS417_01300 and PS417_01305 are separated by 26 nucleotides
PS417_01305 and PS417_01310 overlap by 4 nucleotides
PS417_01290: PS417_01290 - ADP-ribose diphosphatase, at 280,235 to 280,801
_01290
PS417_01295: PS417_01295 - adenosine-3'(2'),5'-bisphosphate nucleotidase, at 280,798 to 281,634
_01295
PS417_01300: PS417_01300 - thioesterase, at 281,761 to 282,216
_01300
PS417_01305: PS417_01305 - Fis family transcriptional regulator, at 282,243 to 283,619
_01305
PS417_01310: PS417_01310 - ATPase, at 283,616 to 285,424
_01310
Group
Condition
PS417
_01290
PS417
_01295
PS417
_01300
PS417
_01305
PS417
_01310
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-2.9
N.D.
-0.7
-0.6
-0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-1.7
N.D.
-1.1
+0.0
-0.9
carbon source
succinate (C)
-1.5
N.D.
+0.1
-1.0
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.6
N.D.
+0.4
-0.6
-0.1
phage
P. simiae Grant Run MOI 0.1
-2.7
N.D.
-0.1
-0.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-3.7
N.D.
+0.7
+0.0
+0.2
carbon source
octanoate (C)
-1.8
N.D.
+0.0
-0.7
-0.1
phage
P. simiae OR Antrim MOI 0.1
-1.4
N.D.
-0.0
-0.1
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.6
N.D.
+0.0
-0.3
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.3
N.D.
-0.0
-1.1
-0.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.6
N.D.
-2.9
+0.3
-0.4
phage
JP1 MOI 1
-1.6
N.D.
+0.8
-0.5
-1.0
motility
inner cut, LB soft agar motility assay
-2.2
N.D.
+0.2
-0.1
+0.0
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.7
N.D.
+0.2
-0.5
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-1.4
N.D.
+0.5
+0.1
-0.9
root
root sample 6; outgrowth in LB
+0.5
N.D.
-1.8
+0.3
-0.6
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.9
N.D.
+0.5
-0.3
+0.1
phage
P. simiae OR Antrim MOI 10
-1.3
N.D.
-0.4
-0.2
+0.6
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.6
N.D.
-0.9
+0.2
-0.8
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+1.1
N.D.
-0.7
-0.3
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
+0.4
-0.5
-1.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-1.3
N.D.
-0.2
+0.4
+0.5
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.8
N.D.
-1.1
+0.2
-0.4
phage
P. simiae ORA MOI 10
-1.4
N.D.
+0.4
+0.1
+0.8
nitrogen source
Adenine (N)
+1.2
N.D.
-0.5
+0.4
-0.5
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
+1.6
N.D.
-0.6
+0.3
-0.1
seeds
Growth on radish seeds for 72 hours
+0.8
N.D.
-0.5
+1.0
+0.4
phage
P. simiae OR1 MOI 0.1
+2.2
N.D.
-0.6
+0.6
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+2.2
N.D.
+1.1
-0.8
+0.0
phage
Wcs_1
+1.9
N.D.
+0.2
+0.6
-0.1
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