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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_12955 and PS417_12960 are separated by 317 nucleotides
PS417_12960 and PS417_12965 are separated by 189 nucleotides
PS417_12965 and PS417_12970 are separated by 59 nucleotides
PS417_12970 and PS417_12975 overlap by 4 nucleotides
PS417_12955: PS417_12955 - sulfonate ABC transporter substrate-binding protein, at 2,831,790 to 2,832,746
_12955
PS417_12960: PS417_12960 - transporter, at 2,833,064 to 2,833,279
_12960
PS417_12965: PS417_12965 - aminotransferase, at 2,833,469 to 2,834,620
_12965
PS417_12970: PS417_12970 - FMN-dependent NADH-azoreductase, at 2,834,680 to 2,835,273
_12970
PS417_12975: PS417_12975 - GCN5 family acetyltransferase, at 2,835,270 to 2,835,710
_12975
Group
Condition
PS417
_12955
PS417
_12960
PS417
_12965
PS417
_12970
PS417
_12975
phage
JP1 MOI 10
-0.9
N.D.
-0.4
-0.8
+0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.2
N.D.
-0.5
-0.4
-0.6
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
+0.1
N.D.
-0.2
-0.6
-0.5
phage
Wcs_1
-0.4
N.D.
-0.7
+0.2
-0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.2
N.D.
+0.2
-0.5
-0.4
phage
JP1 MOI 0.1
+0.3
N.D.
-0.6
-0.4
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.0
N.D.
-0.4
+0.3
-0.8
phage
JP1 MOI 1
-0.2
N.D.
+0.4
-0.0
-0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.0
N.D.
-0.2
-0.8
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.8
N.D.
+0.3
+0.3
+0.1
nophagecontrol
Only library
-0.1
N.D.
-0.6
+0.7
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.8
N.D.
-0.2
-0.4
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
N.D.
-0.3
+0.5
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.9
N.D.
-0.1
-0.2
-0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.2
N.D.
-0.5
-0.1
+0.7
nophagecontrol
TIme6-nophage
-0.1
N.D.
-0.5
+0.5
+0.6
seeds
Growth on radish seeds for 72 hours
+0.7
N.D.
+0.1
-0.5
+0.2
carbon source
L-Valine (C)
+0.8
N.D.
-0.3
+0.1
-0.1
soil
soil sample 6; outgrowth in LB
-0.0
N.D.
+0.3
-0.4
+0.6
seeds
Growth on radish seeds for 72 hours
+0.9
N.D.
-0.2
-0.7
+0.5
solid stress
Fraxetin 3 mM; solid stress
+0.3
N.D.
-0.5
+0.0
+0.7
phage
P. simiae ORA MOI 1
+0.4
N.D.
+0.4
+0.3
-0.4
carbon source
2'-Deoxyinosine 5 mM (C)
-0.4
N.D.
+0.4
+0.7
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
N.D.
-0.3
+0.5
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.7
N.D.
+0.1
-0.2
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
-0.3
+0.4
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
N.D.
+0.6
+0.2
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
N.D.
-0.0
+0.8
+0.4
carbon source
Trisodium citrate (C)
+0.1
N.D.
+0.7
+0.5
+0.5
phage
P. simiae OR Antrim MOI 1
+0.5
N.D.
+0.4
+0.5
+0.4
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