Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_12955 and PS417_12960 are separated by 317 nucleotidesPS417_12960 and PS417_12965 are separated by 189 nucleotidesPS417_12965 and PS417_12970 are separated by 59 nucleotidesPS417_12970 and PS417_12975 overlap by 4 nucleotides PS417_12955: PS417_12955 - sulfonate ABC transporter substrate-binding protein, at 2,831,790 to 2,832,746 _12955 PS417_12960: PS417_12960 - transporter, at 2,833,064 to 2,833,279 _12960 PS417_12965: PS417_12965 - aminotransferase, at 2,833,469 to 2,834,620 _12965 PS417_12970: PS417_12970 - FMN-dependent NADH-azoreductase, at 2,834,680 to 2,835,273 _12970 PS417_12975: PS417_12975 - GCN5 family acetyltransferase, at 2,835,270 to 2,835,710 _12975
Group Condition PS417_12955 PS417_12960 PS417_12965 PS417_12970 PS417_12975
phage JP1 MOI 10 -0.9 N.D. -0.4 -0.8 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 N.D. -0.5 -0.4 -0.6
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.1 N.D. -0.2 -0.6 -0.5
phage Wcs_1 -0.4 N.D. -0.7 +0.2 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 N.D. +0.2 -0.5 -0.4
phage JP1 MOI 0.1 +0.3 N.D. -0.6 -0.4 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.0 N.D. -0.4 +0.3 -0.8
phage JP1 MOI 1 -0.2 N.D. +0.4 -0.0 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.0 N.D. -0.2 -0.8 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 N.D. +0.3 +0.3 +0.1
nophagecontrol Only library -0.1 N.D. -0.6 +0.7 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.8 N.D. -0.2 -0.4 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 N.D. -0.3 +0.5 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.9 N.D. -0.1 -0.2 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 N.D. -0.5 -0.1 +0.7
nophagecontrol TIme6-nophage -0.1 N.D. -0.5 +0.5 +0.6
seeds Growth on radish seeds for 72 hours +0.7 N.D. +0.1 -0.5 +0.2
carbon source L-Valine (C) +0.8 N.D. -0.3 +0.1 -0.1
soil soil sample 6; outgrowth in LB -0.0 N.D. +0.3 -0.4 +0.6
seeds Growth on radish seeds for 72 hours +0.9 N.D. -0.2 -0.7 +0.5
solid stress Fraxetin 3 mM; solid stress +0.3 N.D. -0.5 +0.0 +0.7
phage P. simiae ORA MOI 1 +0.4 N.D. +0.4 +0.3 -0.4
carbon source 2'-Deoxyinosine 5 mM (C) -0.4 N.D. +0.4 +0.7 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 N.D. -0.3 +0.5 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.7 N.D. +0.1 -0.2 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 N.D. -0.3 +0.4 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 N.D. +0.6 +0.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 N.D. -0.0 +0.8 +0.4
carbon source Trisodium citrate (C) +0.1 N.D. +0.7 +0.5 +0.5
phage P. simiae OR Antrim MOI 1 +0.5 N.D. +0.4 +0.5 +0.4
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