Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01285 and PS417_01290 are separated by 87 nucleotidesPS417_01290 and PS417_01295 overlap by 4 nucleotidesPS417_01295 and PS417_01300 are separated by 126 nucleotidesPS417_01300 and PS417_01305 are separated by 26 nucleotides PS417_01285: PS417_01285 - haloacid dehalogenase, at 279,485 to 280,147 _01285 PS417_01290: PS417_01290 - ADP-ribose diphosphatase, at 280,235 to 280,801 _01290 PS417_01295: PS417_01295 - adenosine-3'(2'),5'-bisphosphate nucleotidase, at 280,798 to 281,634 _01295 PS417_01300: PS417_01300 - thioesterase, at 281,761 to 282,216 _01300 PS417_01305: PS417_01305 - Fis family transcriptional regulator, at 282,243 to 283,619 _01305
Group Condition PS417_01285 PS417_01290 PS417_01295 PS417_01300 PS417_01305
soil soil sample 5; outgrowth in LB -4.2 +0.1 N.D. -1.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 -2.9 N.D. -0.7 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -2.6 N.D. +0.4 -0.6
liquid stress 80% Methanol 2 mM; liquid stress -1.8 +0.1 N.D. -1.0 -0.1
phage P. simiae Grant Run MOI 0.1 +0.2 -2.7 N.D. -0.1 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -1.3 N.D. -0.0 -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.6 -1.0 N.D. -0.8 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.0 -1.4 N.D. +0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -1.7 N.D. +0.2 -0.5
carbon source succinate (C) +0.5 -1.5 N.D. +0.1 -1.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 +0.6 N.D. -2.9 +0.3
solid stress Fraxetin 3 mM; solid stress +0.5 -1.2 N.D. -0.7 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.4 -3.7 N.D. +0.7 +0.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.4 +1.1 N.D. -0.7 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +1.4 -1.7 N.D. -1.1 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 -1.7 N.D. -0.3 -0.1
phage JP1 MOI 1 +0.3 -1.6 N.D. +0.8 -0.5
motility inner cut, LB soft agar motility assay +1.2 -2.2 N.D. +0.2 -0.1
root root sample 6; outgrowth in LB +0.2 +0.5 N.D. -1.8 +0.3
supernatant control Vogels_fungal_media 0.1X -0.7 +0.5 N.D. -1.2 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.5 +1.1 N.D. +0.1 +0.0
seeds Growth on radish seeds for 24 hours +0.7 -0.6 N.D. -0.7 +0.8
seeds Growth on radish seeds for 72 hours -1.0 +0.8 N.D. -0.5 +1.0
agar plate interaction control Taped volatile agar plate with no fungus -1.3 +1.1 N.D. +0.3 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 -0.8 N.D. +0.6 +0.6
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.7 -0.7 N.D. +0.3 +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.3 +1.6 N.D. -0.6 +0.3
phage P. simiae OR1 MOI 0.1 +0.4 +2.2 N.D. -0.6 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +2.2 N.D. +1.1 -0.8
phage Wcs_1 +0.9 +1.9 N.D. +0.2 +0.6
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