Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01270 and PS417_01275 are separated by 97 nucleotidesPS417_01275 and PS417_01280 are separated by 132 nucleotidesPS417_01280 and PS417_01285 are separated by 141 nucleotidesPS417_01285 and PS417_01290 are separated by 87 nucleotides PS417_01270: PS417_01270 - iron ABC transporter substrate-binding protein, at 276,946 to 277,785 _01270 PS417_01275: PS417_01275 - LysR family transcriptional regulator, at 277,883 to 278,770 _01275 PS417_01280: PS417_01280 - peptidoglycan-binding protein LysM, at 278,903 to 279,343 _01280 PS417_01285: PS417_01285 - haloacid dehalogenase, at 279,485 to 280,147 _01285 PS417_01290: PS417_01290 - ADP-ribose diphosphatase, at 280,235 to 280,801 _01290
Group Condition PS417_01270 PS417_01275 PS417_01280 PS417_01285 PS417_01290
soil soil sample 5; outgrowth in LB +0.3 -0.3 -1.4 -4.2 +0.1
solid stress Fraxetin 2 mM; solid stress -4.7 -0.5 -0.1 -0.1 +0.5
phage P. simiae Grant Run MOI 0.1 -1.3 +0.1 -0.7 +0.2 -2.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.2 -0.3 +0.4 -0.5 -2.6
solid stress Fraxetin 3 mM; solid stress -1.6 -0.9 -0.0 -0.3 -1.1
solid stress Fraxetin 3 mM; solid stress -1.2 -1.6 -0.4 +0.5 -1.2
carbon source octanoate (C) -0.9 -0.5 -0.5 -0.1 -1.8
rhizosphere rhizosphere sample 2; outgrowth in LB -0.6 -1.0 -0.7 -1.1 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.3 -0.3 +0.1 -0.4 -1.7
stress Gentamicin 0.008 mg/ml -1.8 +0.0 +1.0 -0.9 -1.0
phage P. simiae ORA MOI 10 +0.5 -0.4 -0.6 -0.4 -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.2 +0.2 +0.8 -1.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.7 -0.6 +0.4 +0.3 -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 +0.0 -0.4 +1.1 -1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.1 +0.6 +1.4 -3.7
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.9 +0.7 -1.4 +1.1
motility inner cut, LB soft agar motility assay +0.1 -0.1 +0.1 +1.2 -2.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.1 -0.9 -1.0 -0.2 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 +0.3 +0.3 -1.5 +1.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.6 -0.6 +0.2 +1.4 -1.7
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.8 -0.3 +1.9 -0.4 -0.2
phage P. simiae OR1 MOI 0.1 -0.4 -0.5 -0.5 +0.4 +2.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.8 -0.0 -0.1 +0.0 -1.4
stress Neomycin 0.04 mg/ml -0.4 -0.3 +0.4 -0.4 +2.1
agar plate interaction control Taped volatile agar plate with no fungus +0.7 -0.5 +1.4 -1.3 +1.1
phage Wcs_1 -0.1 +0.0 -0.9 +0.9 +1.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.1 +0.8 +1.7 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 +0.8 +0.3 +0.2 +2.2
carbon source L-Valine (C) +1.6 +0.6 +0.9 -0.9 +1.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +8.0 +0.5 +0.2 -0.5 -2.9
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