Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_12570 and PS417_12580 are separated by 1430 nucleotidesPS417_12580 and PS417_12585 are separated by 151 nucleotidesPS417_12585 and PS417_12590 are separated by 6 nucleotidesPS417_12590 and PS417_12595 are separated by 287 nucleotides PS417_12570: PS417_12570 - AP endonuclease, at 2,734,928 to 2,735,710 _12570 PS417_12580: PS417_12580 - membrane protein, at 2,737,141 to 2,737,782 _12580 PS417_12585: PS417_12585 - acriflavine resistance protein B, at 2,737,934 to 2,741,026 _12585 PS417_12590: PS417_12590 - RND transporter, at 2,741,033 to 2,742,175 _12590 PS417_12595: PS417_12595 - transcriptional regulator, at 2,742,463 to 2,743,164 _12595
Group Condition PS417_12570 PS417_12580 PS417_12585 PS417_12590 PS417_12595
stress Spectinomycin 0.4 mg/ml -0.2 -0.0 -3.7 N.D. -4.7
stress Polymyxin B sulfate 0.006 mg/ml +0.4 -0.3 -5.9 N.D. -2.7
solid stress Fraxetin 3 mM; solid stress -0.1 -0.1 -0.9 N.D. -4.4
stress Gentamicin 0.008 mg/ml +0.6 -0.1 -2.4 N.D. -2.2
stress Doxycycline hyclate 0.001 mg/ml +0.3 -0.2 +1.2 N.D. -4.4
phage P. simiae Grant Run MOI 10 -0.5 -0.0 -0.1 N.D. -2.4
stress Tetracycline 0.001 mg/ml +0.0 +0.1 +1.3 N.D. -4.4
stress Neomycin 0.04 mg/ml +0.0 +0.1 -0.9 N.D. -2.1
solid stress Fraxetin 2 mM; solid stress +0.2 -0.0 -0.0 N.D. -3.0
solid stress Fraxetin 3 mM; solid stress +0.2 -0.1 -0.9 N.D. -1.8
no stress control on plate growth on LB_noSalt plate -0.1 +0.2 -1.4 N.D. -1.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.0 -0.3 +0.3 N.D. -2.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.7 -0.4 -3.4 N.D. +2.3
stress Nalidixic 0.015 mg/ml -0.3 +0.0 +1.8 N.D. -2.8
carbon source L-Valine (C) -0.1 +0.1 +0.8 N.D. -1.8
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 -0.4 -2.7 N.D. +2.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 -0.2 -1.1 N.D. +1.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 -0.4 -1.9 N.D. +2.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 -0.2 -1.8 N.D. +2.1
stress Nalidixic 0.025 mg/ml -0.2 +0.2 +2.6 N.D. -2.5
stress methylglyoxal 0.032 vol% -0.2 -0.3 +1.5 N.D. -0.9
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.6 +0.0 -2.4 N.D. +2.3
soil soil sample 4; outgrowth in LB -0.5 -0.4 +0.6 N.D. +1.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 -0.5 -0.2 N.D. +1.5
soil soil sample 2; outgrowth in LB +0.0 +0.5 +0.0 N.D. +2.5
rhizosphere rhizosphere sample 2; outgrowth in LB -1.4 +0.3 +0.7 N.D. +4.1
root root sample 6; outgrowth in LB +0.2 +0.3 +0.7 N.D. +2.6
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.2 +0.1 +2.3 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +4.0 -0.1 +0.6 N.D. +0.1
rhizosphere rhizosphere sample 8; outgrowth in LB +0.2 +0.2 +0.4 N.D. +4.8
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