Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_12565 and PS417_12570 overlap by 8 nucleotidesPS417_12570 and PS417_12580 are separated by 1430 nucleotidesPS417_12580 and PS417_12585 are separated by 151 nucleotidesPS417_12585 and PS417_12590 are separated by 6 nucleotides PS417_12565: PS417_12565 - 2-dehydro-3-deoxygluconokinase, at 2,733,994 to 2,734,935 _12565 PS417_12570: PS417_12570 - AP endonuclease, at 2,734,928 to 2,735,710 _12570 PS417_12580: PS417_12580 - membrane protein, at 2,737,141 to 2,737,782 _12580 PS417_12585: PS417_12585 - acriflavine resistance protein B, at 2,737,934 to 2,741,026 _12585 PS417_12590: PS417_12590 - RND transporter, at 2,741,033 to 2,742,175 _12590
Group Condition PS417_12565 PS417_12570 PS417_12580 PS417_12585 PS417_12590
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -5.0 -0.7 +0.1 +0.2 N.D.
stress Polymyxin B sulfate 0.006 mg/ml +0.4 +0.4 -0.3 -5.9 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 -0.7 -0.4 -3.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -3.7 -0.3 -0.3 -0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -4.0 -0.1 +0.4 -0.1 N.D.
stress Spectinomycin 0.4 mg/ml +0.2 -0.2 -0.0 -3.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.7 -0.0 -0.2 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.0 -0.5 -0.6 -0.2 N.D.
phage P. simiae ORA MOI 1 -1.7 -0.6 -0.3 -0.4 N.D.
motility inner cut, LB soft agar motility assay -1.6 -0.4 -0.1 -0.8 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.0 +0.6 +0.0 -2.4 N.D.
phage JP1 MOI 0.1 -1.2 -0.3 -0.6 -0.6 N.D.
nitrogen source Urea (N) -1.5 -1.1 +0.2 -0.1 N.D.
no phage control Time6-nophage -2.3 -0.6 +0.4 +0.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.2 -1.1 +0.6 +0.3 N.D.
stress Gentamicin 0.008 mg/ml -0.3 +0.6 -0.1 -2.4 N.D.
phage P. simiae ORA MOI 10 -1.2 -0.6 +0.3 -0.5 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.9 +0.1 -0.4 -2.7 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.4 +0.1 -0.4 -1.9 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 +0.3 +0.3 -1.9 N.D.
stress methylglyoxal 0.032 vol% -0.8 -0.2 -0.3 +1.5 N.D.
stress Nalidixic 0.015 mg/ml -0.5 -0.3 +0.0 +1.8 N.D.
rhizosphere rhizosphere sample 2; outgrowth in LB +2.3 -1.4 +0.3 +0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.4 +1.1 -0.5 +0.0 N.D.
stress Nalidixic 0.025 mg/ml +0.2 -0.2 +0.2 +2.6 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB +1.8 +0.6 -0.2 +1.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3 +1.2 +0.1 +2.3 N.D.
rhizosphere rhizosphere sample 8; outgrowth in LB +2.6 +0.2 +0.2 +0.4 N.D.
root root sample 6; outgrowth in LB +2.7 +0.2 +0.3 +0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.6 +4.0 -0.1 +0.6 N.D.
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