Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_12520 and PS417_12525 are separated by 163 nucleotidesPS417_12525 and PS417_12530 overlap by 8 nucleotidesPS417_12530 and PS417_12535 are separated by 81 nucleotidesPS417_12535 and PS417_12540 are separated by 39 nucleotides PS417_12520: PS417_12520 - hypothetical protein, at 2,725,116 to 2,725,436 _12520 PS417_12525: PS417_12525 - hypothetical protein, at 2,725,600 to 2,725,854 _12525 PS417_12530: PS417_12530 - LysR family transcriptional regulator, at 2,725,847 to 2,726,740 _12530 PS417_12535: PS417_12535 - nitrilase, at 2,726,822 to 2,727,748 _12535 PS417_12540: PS417_12540 - hypothetical protein, at 2,727,788 to 2,728,249 _12540
Group Condition PS417_12520 PS417_12525 PS417_12530 PS417_12535 PS417_12540
soil soil sample 5; outgrowth in LB -1.0 -0.5 +1.1 -4.2 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.4 +0.2 -0.2 -0.0 -1.9
phage JP1 MOI 10 -1.4 +0.2 -0.1 -0.3 -2.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.0 +0.1 -0.5 -0.8 -1.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.8 -0.9 -1.7 -0.0
carbon source 2'-Deoxyinosine 5 mM (C) +0.3 -2.3 +0.1 +0.1 -0.5
phage JP1 MOI 1 -1.1 +0.4 -0.6 -0.4 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.7 -0.3 -1.5 +1.0
stress methylglyoxal 0.032 vol% +0.5 -0.3 -0.2 -0.8 -1.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.7 -1.0 -0.4 -0.3 +1.3
nophagecontrol Only library -0.0 +0.5 -0.2 -1.5 -0.7
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 -0.9 -0.1 -0.3 -1.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.3 +0.5 +0.4 -0.7 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.8 +0.1 -0.5 +0.7 -0.2
carbon source 2'-Deoxyinosine 5 mM (C) -0.4 -1.1 +0.7 +0.0 -0.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.3 -0.1 +0.5 -1.1 -1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.1 -0.8 -0.2 +0.4 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.4 -0.2 -1.2 +0.8
soil soil sample 7; outgrowth in LB -1.3 -0.2 +0.8 +0.6 -0.4
stress R2A with Polymyxin B sulfate 0.002 mg/ml +1.6 -0.7 -0.4 -1.1 +0.2
motility inner cut, LB soft agar motility assay -1.1 -0.5 +0.1 +0.7 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.2 -0.2 -0.2 +0.2 -1.1
phage Wcs_1 +0.1 -0.7 -0.4 +0.5 +1.3
rhizosphere rhizosphere sample 2; outgrowth in LB -0.3 -0.6 +1.8 +0.3 -0.3
root root sample 6; outgrowth in LB -1.0 -0.5 +1.7 +0.5 +0.4
soil soil sample 4; outgrowth in LB -0.6 +0.7 +1.0 +0.2 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 +1.8 +0.6 -0.4 +0.4
rhizosphere rhizosphere sample 1; outgrowth in LB -0.3 -0.0 +1.5 +0.3 +0.9
soil soil sample 8; outgrowth in LB -0.3 +0.4 +1.0 +1.1 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +3.1 +0.1 +0.2 +0.2 -0.1
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