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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_12410 and PS417_12415 are separated by 15 nucleotides
PS417_12415 and PS417_12420 are separated by 171 nucleotides
PS417_12420 and PS417_12425 are separated by 101 nucleotides
PS417_12425 and PS417_12430 are separated by 145 nucleotides
PS417_12410: PS417_12410 - ketosteroid isomerase, at 2,699,524 to 2,699,859
_12410
PS417_12415: PS417_12415 - ATPase, at 2,699,875 to 2,700,978
_12415
PS417_12420: PS417_12420 - GNAT family acetyltransferase, at 2,701,150 to 2,701,641
_12420
PS417_12425: PS417_12425 - hypothetical protein, at 2,701,743 to 2,701,964
_12425
PS417_12430: PS417_12430 - TonB-dependent receptor, at 2,702,110 to 2,704,338
_12430
Group
Condition
PS417
_12410
PS417
_12415
PS417
_12420
PS417
_12425
PS417
_12430
no phage control
Time6-nophage
N.D.
-0.4
N.D.
N.D.
-0.2
solid stress
Fraxetin 1 mM; solid stress
N.D.
-0.2
N.D.
N.D.
-0.3
solid stress
Fraxetin 1 mM; solid stress
N.D.
-0.1
N.D.
N.D.
-0.3
phage
P. simiae OR Antrim MOI 0.1
N.D.
-0.3
N.D.
N.D.
-0.1
motility
outer cut, LB soft agar motility assay
N.D.
-0.3
N.D.
N.D.
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-0.5
N.D.
N.D.
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.5
N.D.
N.D.
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
-0.6
N.D.
N.D.
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
-0.4
N.D.
N.D.
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-0.3
N.D.
N.D.
+0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.3
N.D.
N.D.
+0.3
seeds
Growth on radish seeds for 72 hours
N.D.
+0.2
N.D.
N.D.
-0.2
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
-0.2
N.D.
N.D.
+0.3
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
-0.3
N.D.
N.D.
+0.4
phage
JP1 MOI 1
N.D.
+0.4
N.D.
N.D.
-0.2
seeds
Growth on radish seeds for 72 hours
N.D.
+0.3
N.D.
N.D.
-0.2
phage
JP1 MOI 10
N.D.
+0.3
N.D.
N.D.
-0.2
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
-0.2
N.D.
N.D.
+0.4
seeds
Growth on radish seeds for 72 hours
N.D.
+0.4
N.D.
N.D.
-0.1
rhizosphere
rhizosphere sample 8; outgrowth in LB
N.D.
+0.5
N.D.
N.D.
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.3
N.D.
N.D.
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.3
N.D.
N.D.
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.1
N.D.
N.D.
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
N.D.
+0.4
N.D.
N.D.
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.4
N.D.
N.D.
+0.2
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
+0.2
N.D.
N.D.
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
+0.6
N.D.
N.D.
+0.0
stress
methylglyoxal 0.032 vol%
N.D.
+0.3
N.D.
N.D.
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
+0.5
N.D.
N.D.
+0.1
phage
JP1 MOI 10
N.D.
+0.8
N.D.
N.D.
+0.1
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