Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_12410 and PS417_12415 are separated by 15 nucleotidesPS417_12415 and PS417_12420 are separated by 171 nucleotidesPS417_12420 and PS417_12425 are separated by 101 nucleotidesPS417_12425 and PS417_12430 are separated by 145 nucleotides PS417_12410: PS417_12410 - ketosteroid isomerase, at 2,699,524 to 2,699,859 _12410 PS417_12415: PS417_12415 - ATPase, at 2,699,875 to 2,700,978 _12415 PS417_12420: PS417_12420 - GNAT family acetyltransferase, at 2,701,150 to 2,701,641 _12420 PS417_12425: PS417_12425 - hypothetical protein, at 2,701,743 to 2,701,964 _12425 PS417_12430: PS417_12430 - TonB-dependent receptor, at 2,702,110 to 2,704,338 _12430
Group Condition PS417_12410 PS417_12415 PS417_12420 PS417_12425 PS417_12430
no phage control Time6-nophage N.D. -0.4 N.D. N.D. -0.2
solid stress Fraxetin 1 mM; solid stress N.D. -0.2 N.D. N.D. -0.3
solid stress Fraxetin 1 mM; solid stress N.D. -0.1 N.D. N.D. -0.3
phage P. simiae OR Antrim MOI 0.1 N.D. -0.3 N.D. N.D. -0.1
motility outer cut, LB soft agar motility assay N.D. -0.3 N.D. N.D. -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.5 N.D. N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 N.D. N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.6 N.D. N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.4 N.D. N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 N.D. N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 N.D. N.D. +0.3
seeds Growth on radish seeds for 72 hours N.D. +0.2 N.D. N.D. -0.2
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.2 N.D. N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.3 N.D. N.D. +0.4
phage JP1 MOI 1 N.D. +0.4 N.D. N.D. -0.2
seeds Growth on radish seeds for 72 hours N.D. +0.3 N.D. N.D. -0.2
phage JP1 MOI 10 N.D. +0.3 N.D. N.D. -0.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -0.2 N.D. N.D. +0.4
seeds Growth on radish seeds for 72 hours N.D. +0.4 N.D. N.D. -0.1
rhizosphere rhizosphere sample 8; outgrowth in LB N.D. +0.5 N.D. N.D. -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 N.D. N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 N.D. N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.1 N.D. N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.4 N.D. N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 N.D. N.D. +0.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. +0.2 N.D. N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.6 N.D. N.D. +0.0
stress methylglyoxal 0.032 vol% N.D. +0.3 N.D. N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.5 N.D. N.D. +0.1
phage JP1 MOI 10 N.D. +0.8 N.D. N.D. +0.1
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