Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_12320 and PS417_12325 are separated by 65 nucleotidesPS417_12325 and PS417_12330 are separated by 70 nucleotidesPS417_12330 and PS417_12335 overlap by 4 nucleotidesPS417_12335 and PS417_12340 are separated by 11 nucleotides PS417_12320: PS417_12320 - amino acid ABC transporter substrate-binding protein, at 2,679,506 to 2,680,090 _12320 PS417_12325: PS417_12325 - precorrin-4 C11-methyltransferase, at 2,680,156 to 2,680,902 _12325 PS417_12330: PS417_12330 - cobalamin biosynthesis protein CobE, at 2,680,973 to 2,681,365 _12330 PS417_12335: PS417_12335 - cobalt transporter, at 2,681,362 to 2,682,066 _12335 PS417_12340: PS417_12340 - cobalt transporter, at 2,682,078 to 2,682,266 _12340
Group Condition PS417_12320 PS417_12325 PS417_12330 PS417_12335 PS417_12340
nitrogen source Adenosine (N) N.D. -2.5 N.D. -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -1.1 N.D. -0.8 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.5 N.D. -0.3 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 N.D. -1.1 N.D.
ecofab LB in EcoFAB_3.5mL N.D. -1.2 N.D. -0.3 N.D.
soil soil sample 2; outgrowth in LB N.D. -1.3 N.D. -0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.2 N.D. -0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.7 N.D. -0.7 N.D.
supernatant control Vogels_fungal_media; pH 5.5 N.D. -1.0 N.D. -0.4 N.D.
soil soil sample 3; outgrowth in LB N.D. -1.2 N.D. -0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.1 N.D. -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.3 N.D. +0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -1.1 N.D. -0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. -1.1 N.D. -0.1 N.D.
phage JP1 MOI 10 N.D. -0.5 N.D. -0.7 N.D.
phage JP1 MOI 1 N.D. +0.1 N.D. -1.3 N.D.
carbon source Xylitol (C) N.D. -0.7 N.D. -0.5 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x N.D. -0.6 N.D. -0.6 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -1.1 N.D. -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -1.0 N.D. -0.2 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.0 N.D. +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.0 N.D. +0.2 N.D.
supernatant control Vogels_fungal_media 0.1X N.D. -1.1 N.D. +0.4 N.D.
phage JP1 MOI 0.1 N.D. +0.4 N.D. -0.8 N.D.
seeds Growth on radish seeds for 72 hours N.D. -0.5 N.D. +1.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.0 N.D. +2.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +1.3 N.D. +0.1 N.D.
stress Gentamicin 0.008 mg/ml N.D. +0.9 N.D. +0.5 N.D.
root root sample 6; outgrowth in LB N.D. -0.6 N.D. +3.1 N.D.
nitrogen source Glycine (N) N.D. +2.7 N.D. +0.7 N.D.
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