Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_11865 and PS417_11870 are separated by 149 nucleotidesPS417_11870 and PS417_11875 are separated by 19 nucleotidesPS417_11875 and PS417_11880 are separated by 155 nucleotidesPS417_11880 and PS417_11885 are separated by 350 nucleotides PS417_11865: PS417_11865 - xylose isomerase, at 2,582,763 to 2,583,584 _11865 PS417_11870: PS417_11870 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (from data), at 2,583,734 to 2,585,665 _11870 PS417_11875: PS417_11875 - Inositol 2-dehydrogenase (EC 1.1.1.18) (from data), at 2,585,685 to 2,586,695 _11875 PS417_11880: PS417_11880 - oxidoreductase, at 2,586,851 to 2,587,879 _11880 PS417_11885: PS417_11885 - Inositol transport system sugar-binding protein (from data), at 2,588,230 to 2,589,159 _11885
Group Condition PS417_11865 PS417_11870 PS417_11875 PS417_11880 PS417_11885
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.4 -4.4 -4.4 +0.3 -5.6
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.2 -4.2 -4.3 +0.5 -4.8
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -3.8 -4.6 -2.8 +0.5 -3.6
carbon source m-Inositol (C) -2.8 -3.0 -3.4 +0.4 -3.4
carbon source m-Inositol (C) -2.9 -3.0 -3.0 +0.3 -3.0
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -3.6 -2.8 -2.3 +0.2 -1.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.2 -2.3 -2.7 +0.5 -2.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.6 -0.8 -0.2 +0.0 -1.3
stress Gentamicin 0.008 mg/ml -0.2 -0.6 -1.1 -1.9 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.6 -0.0 -0.3 -0.8 -1.1
carbon source 2'-Deoxyinosine 5 mM (C) -1.9 +0.1 +0.4 -0.4 +0.4
stress Doxycycline hyclate 0.001 mg/ml +0.5 -0.0 +0.5 -1.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 -0.5 -0.2 +1.1 -0.4
rhizosphere rhizosphere sample 2; outgrowth in LB -2.3 -0.0 -0.1 +0.7 +0.9
phage JP1 MOI 10 -1.9 +0.2 +0.2 +0.7 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 +0.4 +0.7 -0.4 -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.0 -0.2 -0.7 -0.2 +0.8
phage P. simiae Grant Run MOI 0.1 -1.4 +0.5 +0.3 +0.9 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.3 -0.7 +0.4 +1.6
phage P. simiae OR Antrim MOI 10 -1.2 +0.5 +0.8 +0.5 +0.4
nophagecontrol Only library +0.7 +0.4 +0.4 +0.9 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.4 +0.0 +1.0 -0.4 +1.9
seeds Growth on radish seeds for 72 hours +1.2 +0.1 +0.3 +1.6 -1.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.5 +0.3 -0.5 +1.2 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.6 +0.6 +0.8 +0.2 +0.9
phage P. simiae OR1 MOI 0.1 +0.9 +0.5 +0.6 +0.8 +0.4
stress Choline chloride 300 mM +0.3 +0.4 +0.8 +1.0 +0.8
phage P. simiae OR Antrim MOI 0.1 -0.0 +0.4 +1.0 +1.4 +0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.3 +0.7 +0.3 +0.8 -0.3
stress 1-ethyl-3-methylimidazolium chloride 200 mM +0.5 +1.0 +1.4 +2.0 +1.1
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