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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_11755 and PS417_11760 are separated by 49 nucleotides
PS417_11760 and PS417_11765 are separated by 309 nucleotides
PS417_11765 and PS417_11770 are separated by 26 nucleotides
PS417_11770 and PS417_11775 overlap by 4 nucleotides
PS417_11755: PS417_11755 - diacetyl reductase, at 2,557,356 to 2,558,135
_11755
PS417_11760: PS417_11760 - Fis family transcriptional regulator, at 2,558,185 to 2,560,050
_11760
PS417_11765: PS417_11765 - ABC transporter substrate-binding protein, at 2,560,360 to 2,561,337
_11765
PS417_11770: PS417_11770 - pyruvate dehydrogenase, at 2,561,364 to 2,562,383
_11770
PS417_11775: PS417_11775 - acetoin dehydrogenase, at 2,562,380 to 2,563,474
_11775
Group
Condition
PS417
_11755
PS417
_11760
PS417
_11765
PS417
_11770
PS417
_11775
soil
soil sample 3; outgrowth in LB
+0.1
-0.7
-0.3
-2.4
+0.2
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
-0.2
-1.0
+0.0
-1.1
-0.5
phage
P. simiae ORA MOI 1
-0.4
-0.7
-0.0
-0.7
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.8
+0.1
-0.6
-0.1
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.6
-0.1
-0.2
-1.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.6
-0.6
-0.1
-0.2
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.5
+0.2
-1.6
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.1
-0.6
-0.3
-1.0
-0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.3
-0.8
+0.1
-1.3
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
-0.2
-0.4
-1.0
+0.5
carbon source
L-Valine (C)
-0.1
+0.4
-0.3
-2.0
+0.4
phage
JP1 MOI 1
+0.7
-0.6
-0.1
-0.9
-0.3
no phage control
no phage control
+0.2
-0.9
-0.2
+0.5
-0.6
phage
P. simiae Grant Run MOI 0.1
-0.4
-1.0
-0.2
+1.0
-0.2
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
+0.6
-0.5
+0.4
-0.6
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.7
-0.7
+0.2
+0.8
-0.4
motility
outer cut, LB soft agar motility assay
-0.3
+0.4
-0.5
+0.6
-0.9
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.3
-0.6
-0.3
+1.0
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+0.5
-0.3
+0.0
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
-0.9
+0.4
+1.0
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.6
-0.6
+1.0
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
+0.2
-0.3
+1.2
-0.6
phage
P. simiae Grant Run MOI 10
-0.4
-1.0
+0.5
+0.4
+0.6
phage
P. simiae Grant Run MOI 0.1
-0.7
-0.4
+0.3
+0.8
+0.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.2
-0.6
+0.4
-0.5
-0.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.2
-0.8
+0.5
+1.0
-0.0
seeds
Growth on radish seeds for 72 hours
-0.5
+0.2
-0.4
+1.2
-0.1
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.2
+0.3
+0.9
+0.6
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.6
+0.1
-0.2
+1.5
-0.1
stress
Gentamicin 0.008 mg/ml
+0.8
+0.1
+0.3
+0.7
+0.4
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