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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_11740 and PS417_11745 are separated by 182 nucleotides
PS417_11745 and PS417_11750 are separated by 3 nucleotides
PS417_11750 and PS417_11755 are separated by 192 nucleotides
PS417_11755 and PS417_11760 are separated by 49 nucleotides
PS417_11740: PS417_11740 - AsnC family transcriptional regulator, at 2,553,904 to 2,554,374
_11740
PS417_11745: PS417_11745 - alkylhydroperoxidase, at 2,554,557 to 2,554,994
_11745
PS417_11750: PS417_11750 - TonB-dependent receptor, at 2,554,998 to 2,557,163
_11750
PS417_11755: PS417_11755 - diacetyl reductase, at 2,557,356 to 2,558,135
_11755
PS417_11760: PS417_11760 - Fis family transcriptional regulator, at 2,558,185 to 2,560,050
_11760
Group
Condition
PS417
_11740
PS417
_11745
PS417
_11750
PS417
_11755
PS417
_11760
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
-0.1
-0.7
-0.8
phage
P. simiae Grant Run MOI 10
N.D.
N.D.
-0.1
-0.4
-1.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
-0.2
-0.6
-0.6
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
N.D.
N.D.
-0.2
-0.2
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.1
+0.1
-1.5
phage
P. simiae Grant Run MOI 0.1
N.D.
N.D.
+0.1
-0.4
-1.0
phage
P. simiae OR Antrim MOI 10
N.D.
N.D.
+0.0
-0.9
-0.6
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.1
-0.7
-0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
-0.2
-0.8
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.1
-0.4
-0.9
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
-0.1
-0.8
-0.4
phage
P. simiae Grant Run MOI 0.1
N.D.
N.D.
-0.1
-0.7
-0.4
phage
P. simiae OR Antrim MOI 1
N.D.
N.D.
+0.1
-0.7
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.3
-0.7
-0.7
phage
P. simiae ORA MOI 1
N.D.
N.D.
+0.2
-0.4
-0.7
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
N.D.
+0.1
+0.1
-1.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
-0.4
-0.6
+0.2
phage
JP1 MOI 1
N.D.
N.D.
-0.2
+0.4
-0.9
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.4
-0.3
-0.8
ecofab
LB 0.5x in EcoFAB_3.5mL
N.D.
N.D.
-0.1
+0.5
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.0
+0.5
-0.7
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
N.D.
N.D.
-0.1
+0.6
-0.6
stress
Cisplatin 0.01 mg/ml
N.D.
N.D.
-0.0
-0.6
+0.6
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
-0.1
-0.7
+0.7
phage
JP1 MOI 1
N.D.
N.D.
+0.1
+0.7
-0.6
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
N.D.
N.D.
+0.2
+0.6
-0.5
seeds
Growth on radish seeds for 72 hours
N.D.
N.D.
+0.1
-0.3
+0.9
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.2
+1.2
-0.6
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
+0.1
+1.1
-0.4
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
N.D.
+0.1
+1.4
-0.1
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