Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_11735 and PS417_11740 are separated by 123 nucleotidesPS417_11740 and PS417_11745 are separated by 182 nucleotidesPS417_11745 and PS417_11750 are separated by 3 nucleotidesPS417_11750 and PS417_11755 are separated by 192 nucleotides PS417_11735: PS417_11735 - methionine gamma-lyase, at 2,552,545 to 2,553,780 _11735 PS417_11740: PS417_11740 - AsnC family transcriptional regulator, at 2,553,904 to 2,554,374 _11740 PS417_11745: PS417_11745 - alkylhydroperoxidase, at 2,554,557 to 2,554,994 _11745 PS417_11750: PS417_11750 - TonB-dependent receptor, at 2,554,998 to 2,557,163 _11750 PS417_11755: PS417_11755 - diacetyl reductase, at 2,557,356 to 2,558,135 _11755
Group Condition PS417_11735 PS417_11740 PS417_11745 PS417_11750 PS417_11755
rhizosphere rhizosphere sample 2; outgrowth in LB -1.0 N.D. N.D. +0.1 -0.5
phage JP1 MOI 10 -1.1 N.D. N.D. -0.2 -0.0
phage P. simiae OR Antrim MOI 10 -0.3 N.D. N.D. +0.0 -0.9
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.3 N.D. N.D. -0.1 -0.7
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 N.D. N.D. -0.1 -0.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.3 N.D. N.D. -0.4 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 N.D. N.D. -0.2 -0.8
phage JP1 MOI 1 -0.5 N.D. N.D. -0.2 +0.4
liquid stress Scopoletin 2 mM; liquid stress +0.3 N.D. N.D. +0.0 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.2 N.D. N.D. +0.3 -0.7
ecofab LB 0.5x in EcoFAB_3.5mL -0.6 N.D. N.D. -0.1 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. N.D. +0.0 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 N.D. N.D. +0.1 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 N.D. N.D. +0.1 -0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 N.D. N.D. +0.2 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.9 N.D. N.D. -0.2 -0.6
solid stress Fraxetin 3 mM; solid stress +0.6 N.D. N.D. -0.1 -0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.9 N.D. N.D. -0.1 -0.5
seeds Growth on radish seeds for 72 hours +0.6 N.D. N.D. +0.3 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 N.D. N.D. +0.4 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.7 N.D. N.D. +0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.6 N.D. N.D. +0.3 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 N.D. N.D. +0.2 +1.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 N.D. N.D. -0.0 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 N.D. N.D. -0.0 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.4 N.D. N.D. +0.1 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 N.D. N.D. +0.2 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 N.D. N.D. +0.4 +0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. N.D. +0.1 +1.1
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.0 N.D. N.D. +0.1 +1.4
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