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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_11735 and PS417_11740 are separated by 123 nucleotides
PS417_11740 and PS417_11745 are separated by 182 nucleotides
PS417_11745 and PS417_11750 are separated by 3 nucleotides
PS417_11750 and PS417_11755 are separated by 192 nucleotides
PS417_11735: PS417_11735 - methionine gamma-lyase, at 2,552,545 to 2,553,780
_11735
PS417_11740: PS417_11740 - AsnC family transcriptional regulator, at 2,553,904 to 2,554,374
_11740
PS417_11745: PS417_11745 - alkylhydroperoxidase, at 2,554,557 to 2,554,994
_11745
PS417_11750: PS417_11750 - TonB-dependent receptor, at 2,554,998 to 2,557,163
_11750
PS417_11755: PS417_11755 - diacetyl reductase, at 2,557,356 to 2,558,135
_11755
Group
Condition
PS417
_11735
PS417
_11740
PS417
_11745
PS417
_11750
PS417
_11755
rhizosphere
rhizosphere sample 2; outgrowth in LB
-1.0
N.D.
N.D.
+0.1
-0.5
phage
JP1 MOI 10
-1.1
N.D.
N.D.
-0.2
-0.0
phage
P. simiae OR Antrim MOI 10
-0.3
N.D.
N.D.
+0.0
-0.9
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.3
N.D.
N.D.
-0.1
-0.7
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
N.D.
N.D.
-0.1
-0.8
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
+0.3
N.D.
N.D.
-0.4
-0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.5
N.D.
N.D.
-0.2
-0.8
phage
JP1 MOI 1
-0.5
N.D.
N.D.
-0.2
+0.4
liquid stress
Scopoletin 2 mM; liquid stress
+0.3
N.D.
N.D.
+0.0
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
N.D.
N.D.
+0.3
-0.7
ecofab
LB 0.5x in EcoFAB_3.5mL
-0.6
N.D.
N.D.
-0.1
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
N.D.
N.D.
+0.0
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
N.D.
N.D.
+0.1
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.6
N.D.
N.D.
+0.1
-0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.4
N.D.
N.D.
+0.2
-0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.9
N.D.
N.D.
-0.2
-0.6
solid stress
Fraxetin 3 mM; solid stress
+0.6
N.D.
N.D.
-0.1
-0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.9
N.D.
N.D.
-0.1
-0.5
seeds
Growth on radish seeds for 72 hours
+0.6
N.D.
N.D.
+0.3
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.4
N.D.
N.D.
+0.4
-0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.7
N.D.
N.D.
+0.1
-0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.6
N.D.
N.D.
+0.3
-0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
N.D.
N.D.
+0.2
+1.2
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.9
N.D.
N.D.
-0.0
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.5
N.D.
N.D.
-0.0
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+1.4
N.D.
N.D.
+0.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
N.D.
+0.2
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
N.D.
N.D.
+0.4
+0.4
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.3
N.D.
N.D.
+0.1
+1.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.0
N.D.
N.D.
+0.1
+1.4
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