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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_11730 and PS417_11735 are separated by 146 nucleotides
PS417_11735 and PS417_11740 are separated by 123 nucleotides
PS417_11740 and PS417_11745 are separated by 182 nucleotides
PS417_11745 and PS417_11750 are separated by 3 nucleotides
PS417_11730: PS417_11730 - PvdJ/PvdD/PvdP-like protein, at 2,550,764 to 2,552,398
_11730
PS417_11735: PS417_11735 - methionine gamma-lyase, at 2,552,545 to 2,553,780
_11735
PS417_11740: PS417_11740 - AsnC family transcriptional regulator, at 2,553,904 to 2,554,374
_11740
PS417_11745: PS417_11745 - alkylhydroperoxidase, at 2,554,557 to 2,554,994
_11745
PS417_11750: PS417_11750 - TonB-dependent receptor, at 2,554,998 to 2,557,163
_11750
Group
Condition
PS417
_11730
PS417
_11735
PS417
_11740
PS417
_11745
PS417
_11750
phage
JP1 MOI 10
-0.2
-1.1
N.D.
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.4
-0.5
N.D.
N.D.
-0.3
soil
soil sample 5; outgrowth in LB
-0.4
-0.6
N.D.
N.D.
-0.1
phage
JP1 MOI 0.1
-0.9
-0.2
N.D.
N.D.
+0.2
phage
P. simiae OR Antrim MOI 1
-0.7
-0.3
N.D.
N.D.
+0.1
phage
P. simiae OR Antrim MOI 0.1
-0.7
+0.2
N.D.
N.D.
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.4
+0.3
N.D.
N.D.
-0.4
ecofab
LB 0.5x in EcoFAB_3.5mL
+0.3
-0.6
N.D.
N.D.
-0.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.6
-1.0
N.D.
N.D.
+0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.7
N.D.
N.D.
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.4
N.D.
N.D.
+0.0
phage
P. simiae Grant Run MOI 10
-0.6
+0.4
N.D.
N.D.
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.5
+0.6
N.D.
N.D.
-0.0
stress
methylglyoxal 0.032 vol%
+0.2
+0.3
N.D.
N.D.
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.5
+0.6
N.D.
N.D.
+0.1
seeds
Growth on radish seeds for 72 hours
-0.6
+0.6
N.D.
N.D.
+0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+0.7
N.D.
N.D.
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.4
+0.6
N.D.
N.D.
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.0
+0.9
N.D.
N.D.
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.4
+0.5
N.D.
N.D.
-0.2
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
+1.4
-0.5
N.D.
N.D.
-0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.8
-0.2
N.D.
N.D.
+0.3
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+1.1
-0.3
N.D.
N.D.
-0.0
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.9
N.D.
N.D.
-0.0
carbon source
L-Valine (C)
+0.3
+0.6
N.D.
N.D.
+0.4
phage
P. simiae OR1 MOI 0.1
+0.6
+0.5
N.D.
N.D.
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.3
+0.6
N.D.
N.D.
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.6
+0.4
N.D.
N.D.
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.9
+0.9
N.D.
N.D.
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.2
+1.4
N.D.
N.D.
+0.1
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