Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_11725 and PS417_11730 are separated by 142 nucleotidesPS417_11730 and PS417_11735 are separated by 146 nucleotidesPS417_11735 and PS417_11740 are separated by 123 nucleotidesPS417_11740 and PS417_11745 are separated by 182 nucleotides PS417_11725: PS417_11725 - peptidase M19, at 2,549,272 to 2,550,621 _11725 PS417_11730: PS417_11730 - PvdJ/PvdD/PvdP-like protein, at 2,550,764 to 2,552,398 _11730 PS417_11735: PS417_11735 - methionine gamma-lyase, at 2,552,545 to 2,553,780 _11735 PS417_11740: PS417_11740 - AsnC family transcriptional regulator, at 2,553,904 to 2,554,374 _11740 PS417_11745: PS417_11745 - alkylhydroperoxidase, at 2,554,557 to 2,554,994 _11745
Group Condition PS417_11725 PS417_11730 PS417_11735 PS417_11740 PS417_11745
phage JP1 MOI 10 -0.4 -0.2 -1.1 N.D. N.D.
nophagecontrol Only library -1.1 -0.1 -0.5 N.D. N.D.
phage Wcs_1 -1.2 -0.2 -0.2 N.D. N.D.
phage P. simiae OR Antrim MOI 1 -0.4 -0.7 -0.3 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.8 +0.1 N.D. N.D.
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -1.3 -0.1 +0.2 N.D. N.D.
phage P. simiae ORA MOI 10 -1.0 +0.4 -0.4 N.D. N.D.
phage JP1 MOI 0.1 +0.3 -0.9 -0.2 N.D. N.D.
phage P. simiae Grant Run MOI 10 -0.6 -0.4 +0.3 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 -0.4 +0.3 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 -0.4 +0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 -0.5 +0.6 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.5 -0.4 -0.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.6 +0.5 -0.3 N.D. N.D.
rhizosphere rhizosphere sample 2; outgrowth in LB +0.2 +0.6 -1.0 N.D. N.D.
stress Gentamicin 0.008 mg/ml +0.7 -0.6 +0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 -0.5 -0.2 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 -0.7 +0.4 N.D. N.D.
seeds Growth on radish seeds for 72 hours +0.3 -0.6 +0.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.5 +0.6 +0.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.2 +0.9 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.1 +0.2 -0.7 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 +0.2 +1.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.6 +0.8 -0.2 N.D. N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.4 +1.1 -0.3 N.D. N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.5 +1.4 -0.5 N.D. N.D.
no phage control Time6-nophage +0.7 +0.3 +0.4 N.D. N.D.
soil soil sample 8; outgrowth in LB +1.0 +0.5 -0.1 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.4 +0.5 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 +0.9 +0.9 N.D. N.D.
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