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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_11725 and PS417_11730 are separated by 142 nucleotides
PS417_11730 and PS417_11735 are separated by 146 nucleotides
PS417_11735 and PS417_11740 are separated by 123 nucleotides
PS417_11740 and PS417_11745 are separated by 182 nucleotides
PS417_11725: PS417_11725 - peptidase M19, at 2,549,272 to 2,550,621
_11725
PS417_11730: PS417_11730 - PvdJ/PvdD/PvdP-like protein, at 2,550,764 to 2,552,398
_11730
PS417_11735: PS417_11735 - methionine gamma-lyase, at 2,552,545 to 2,553,780
_11735
PS417_11740: PS417_11740 - AsnC family transcriptional regulator, at 2,553,904 to 2,554,374
_11740
PS417_11745: PS417_11745 - alkylhydroperoxidase, at 2,554,557 to 2,554,994
_11745
Group
Condition
PS417
_11725
PS417
_11730
PS417
_11735
PS417
_11740
PS417
_11745
phage
JP1 MOI 10
-0.4
-0.2
-1.1
N.D.
N.D.
nophagecontrol
Only library
-1.1
-0.1
-0.5
N.D.
N.D.
phage
Wcs_1
-1.2
-0.2
-0.2
N.D.
N.D.
phage
P. simiae OR Antrim MOI 1
-0.4
-0.7
-0.3
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.8
+0.1
N.D.
N.D.
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-1.3
-0.1
+0.2
N.D.
N.D.
phage
P. simiae ORA MOI 10
-1.0
+0.4
-0.4
N.D.
N.D.
phage
JP1 MOI 0.1
+0.3
-0.9
-0.2
N.D.
N.D.
phage
P. simiae Grant Run MOI 10
-0.6
-0.4
+0.3
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
-0.4
+0.3
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.5
-0.4
+0.3
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.7
-0.5
+0.6
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.5
-0.4
-0.5
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
-0.6
+0.5
-0.3
N.D.
N.D.
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.2
+0.6
-1.0
N.D.
N.D.
stress
Gentamicin 0.008 mg/ml
+0.7
-0.6
+0.1
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
-0.5
-0.2
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
-0.7
+0.4
N.D.
N.D.
seeds
Growth on radish seeds for 72 hours
+0.3
-0.6
+0.6
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.5
+0.6
+0.4
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
+0.2
+0.9
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.1
+0.2
-0.7
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.6
+0.2
+1.4
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.6
+0.8
-0.2
N.D.
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.4
+1.1
-0.3
N.D.
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
+0.5
+1.4
-0.5
N.D.
N.D.
no phage control
Time6-nophage
+0.7
+0.3
+0.4
N.D.
N.D.
soil
soil sample 8; outgrowth in LB
+1.0
+0.5
-0.1
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.5
+0.4
+0.5
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
+0.9
+0.9
N.D.
N.D.
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