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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_01125 and PS417_01130 are separated by 108 nucleotides
PS417_01130 and PS417_01135 are separated by 128 nucleotides
PS417_01135 and PS417_01140 are separated by 14 nucleotides
PS417_01140 and PS417_01145 overlap by 4 nucleotides
PS417_01125: PS417_01125 - cysteine desulfhydrase, at 247,229 to 248,557
_01125
PS417_01130: PS417_01130 - L-glyceraldehyde 3-phosphate reductase, at 248,666 to 249,703
_01130
PS417_01135: PS417_01135 - taurine dioxygenase, at 249,832 to 250,668
_01135
PS417_01140: PS417_01140 - taurine ABC transporter permease, at 250,683 to 251,519
_01140
PS417_01145: PS417_01145 - taurine transporter ATP-binding subunit, at 251,516 to 252,295
_01145
Group
Condition
PS417
_01125
PS417
_01130
PS417
_01135
PS417
_01140
PS417
_01145
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
-0.6
-0.6
-0.5
-0.9
-2.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.3
+0.4
-1.4
-2.6
seeds
Growth on radish seeds for 72 hours
-0.0
-0.5
-0.5
+0.2
-3.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
-1.4
-0.5
-0.2
-1.0
nophagecontrol
Only library
-0.4
-3.2
+0.3
-0.5
+1.0
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.6
-0.4
+0.0
+0.1
-3.1
soil
soil sample 8; outgrowth in LB
-0.0
-0.8
+0.2
+0.3
-2.3
phage
P. simiae ORA MOI 0.1
+0.1
-0.2
-0.2
-0.1
-2.2
phage
P. simiae OR1 MOI 0.1
+0.2
-0.9
-1.1
+0.3
-1.0
phage
P. simiae Grant Run MOI 0.1
+0.3
+0.1
+0.0
-0.4
-2.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.3
-0.3
-0.3
-0.3
-1.6
agar plate interaction control
Taped volatile agar plate with no fungus
-0.2
-1.0
+0.7
-0.5
-0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.0
-0.7
-0.1
+0.6
-1.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.2
-0.4
+0.4
+0.3
-1.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.6
-1.1
-0.8
+0.9
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
-0.2
+0.5
+0.8
-0.2
-1.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-1.1
-0.7
+0.7
+0.8
seeds
Growth on radish seeds for 72 hours
+0.6
-0.3
-0.6
-0.6
+1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
+0.5
+0.1
-0.7
+1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
+0.2
+0.7
-1.1
+0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.4
+0.7
-0.8
+1.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+0.3
+1.3
-0.6
+0.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+1.5
+0.8
-0.2
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
+1.0
+0.7
+0.6
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-0.3
+1.0
+0.4
+0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.4
+1.2
-0.2
-0.2
+1.2
stress
Gentamicin 0.008 mg/ml
+0.3
+0.6
-0.0
-0.5
+1.5
phage
P. simiae OR Antrim MOI 0.1
+0.2
-0.1
-0.5
+0.3
+2.3
phage
JP1 MOI 0.1
+0.1
+0.3
+1.1
-0.1
+1.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.2
+2.2
+1.2
-0.3
+0.4
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