Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_01125 and PS417_01130 are separated by 108 nucleotidesPS417_01130 and PS417_01135 are separated by 128 nucleotidesPS417_01135 and PS417_01140 are separated by 14 nucleotidesPS417_01140 and PS417_01145 overlap by 4 nucleotides PS417_01125: PS417_01125 - cysteine desulfhydrase, at 247,229 to 248,557 _01125 PS417_01130: PS417_01130 - L-glyceraldehyde 3-phosphate reductase, at 248,666 to 249,703 _01130 PS417_01135: PS417_01135 - taurine dioxygenase, at 249,832 to 250,668 _01135 PS417_01140: PS417_01140 - taurine ABC transporter permease, at 250,683 to 251,519 _01140 PS417_01145: PS417_01145 - taurine transporter ATP-binding subunit, at 251,516 to 252,295 _01145
Group Condition PS417_01125 PS417_01130 PS417_01135 PS417_01140 PS417_01145
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.6 -0.5 -0.9 -2.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 +0.3 +0.4 -1.4 -2.6
seeds Growth on radish seeds for 72 hours -0.0 -0.5 -0.5 +0.2 -3.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -1.4 -0.5 -0.2 -1.0
nophagecontrol Only library -0.4 -3.2 +0.3 -0.5 +1.0
rhizosphere rhizosphere sample 2; outgrowth in LB +0.6 -0.4 +0.0 +0.1 -3.1
soil soil sample 8; outgrowth in LB -0.0 -0.8 +0.2 +0.3 -2.3
phage P. simiae ORA MOI 0.1 +0.1 -0.2 -0.2 -0.1 -2.2
phage P. simiae OR1 MOI 0.1 +0.2 -0.9 -1.1 +0.3 -1.0
phage P. simiae Grant Run MOI 0.1 +0.3 +0.1 +0.0 -0.4 -2.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.3 -0.3 -0.3 -0.3 -1.6
agar plate interaction control Taped volatile agar plate with no fungus -0.2 -1.0 +0.7 -0.5 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 -0.7 -0.1 +0.6 -1.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -0.4 +0.4 +0.3 -1.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.6 -1.1 -0.8 +0.9
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -0.2 +0.5 +0.8 -0.2 -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -1.1 -0.7 +0.7 +0.8
seeds Growth on radish seeds for 72 hours +0.6 -0.3 -0.6 -0.6 +1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +0.5 +0.1 -0.7 +1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.2 +0.7 -1.1 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.4 +0.7 -0.8 +1.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.3 +1.3 -0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +1.5 +0.8 -0.2 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 +1.0 +0.7 +0.6 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.3 +1.0 +0.4 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 +1.2 -0.2 -0.2 +1.2
stress Gentamicin 0.008 mg/ml +0.3 +0.6 -0.0 -0.5 +1.5
phage P. simiae OR Antrim MOI 0.1 +0.2 -0.1 -0.5 +0.3 +2.3
phage JP1 MOI 0.1 +0.1 +0.3 +1.1 -0.1 +1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 +2.2 +1.2 -0.3 +0.4
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