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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_11305 and PS417_11310 are separated by 329 nucleotides
PS417_11310 and PS417_11315 are separated by 183 nucleotides
PS417_11315 and PS417_11320 are separated by 75 nucleotides
PS417_11320 and PS417_11325 are separated by 67 nucleotides
PS417_11305: PS417_11305 - general secretion pathway protein GspF, at 2,420,879 to 2,422,090
_11305
PS417_11310: PS417_11310 - alkaline phosphatase, at 2,422,420 to 2,423,601
_11310
PS417_11315: PS417_11315 - hemagglutinin, at 2,423,785 to 2,436,357
_11315
PS417_11320: PS417_11320 - hypothetical protein, at 2,436,433 to 2,438,019
_11320
PS417_11325: PS417_11325 - hypothetical protein, at 2,438,087 to 2,439,820
_11325
Group
Condition
PS417
_11305
PS417
_11310
PS417
_11315
PS417
_11320
PS417
_11325
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.4
+0.0
-1.3
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
-0.2
-0.1
-0.6
-0.5
seeds
Growth on radish seeds for 72 hours
-1.3
+0.3
+0.1
-0.7
-0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.3
-0.2
-0.2
-1.1
+0.1
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x
+0.1
+0.2
+0.1
-1.1
-0.4
seeds
Growth on radish seeds for 72 hours
-0.4
-0.2
+0.2
-0.9
+0.2
soil
soil sample 6; outgrowth in LB
-0.6
-0.3
+0.2
-0.5
+0.3
carbon source
L-Valine (C)
-1.0
-0.5
+0.1
+0.4
+0.0
soil
soil sample 5; outgrowth in LB
-0.4
-1.0
+0.2
-0.0
+0.3
phage
JP1 MOI 1
+0.0
-0.5
-0.3
+0.6
-0.6
phage
P. simiae OR Antrim MOI 0.1
-0.8
+0.6
+0.1
-0.5
-0.1
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
-0.5
-0.6
+0.0
+0.6
-0.2
soil
soil sample 8; outgrowth in LB
-0.9
-0.2
+0.1
-0.2
+0.4
phage
P. simiae ORA MOI 1
-0.6
-0.3
-0.1
+0.7
-0.2
rhizosphere
rhizosphere sample 8; outgrowth in LB
-0.1
-0.8
+0.2
-0.1
+0.4
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
+0.4
-0.0
-1.5
+0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
+0.1
-0.1
-0.8
+0.1
stress
Doxycycline hyclate 0.001 mg/ml
+0.5
-0.7
+0.1
-0.3
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.6
+0.0
+0.1
+0.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.4
-0.2
+0.2
+1.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.6
+0.5
-0.1
+0.4
+0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.5
-0.1
+0.1
-0.6
-0.2
stress
methylglyoxal 0.032 vol%
+0.8
-0.4
+0.0
+0.7
-0.4
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.6
-0.4
+0.2
+0.1
+0.5
phage
JP1 MOI 10
+1.0
-0.1
+0.0
-0.2
+0.6
phage
P. simiae OR Antrim MOI 0.1
-0.8
+0.9
+0.1
+0.9
+0.3
soil
soil sample 7; outgrowth in LB
+0.8
-0.2
+0.2
+0.6
+0.1
phage
P. simiae ORA MOI 10
+0.3
-0.1
-0.1
+1.6
-0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.5
+0.1
-0.0
+1.4
-0.1
root
root sample 6; outgrowth in LB
+0.3
+0.2
+0.5
+0.3
+0.7
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