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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_11265 and PS417_11270 overlap by 14 nucleotides
PS417_11270 and PS417_11275 are separated by 148 nucleotides
PS417_11275 and PS417_11280 are separated by 8 nucleotides
PS417_11280 and PS417_11285 overlap by 4 nucleotides
PS417_11265: PS417_11265 - general secretion pathway protein GspC, at 2,412,995 to 2,413,426
_11265
PS417_11270: PS417_11270 - general secretion pathway protein GspI, at 2,413,413 to 2,413,778
_11270
PS417_11275: PS417_11275 - general secretion pathway protein GspG, at 2,413,927 to 2,414,376
_11275
PS417_11280: PS417_11280 - HxcX atypical pseudopilin, at 2,414,385 to 2,415,254
_11280
PS417_11285: PS417_11285 - general secretion pathway protein GspL, at 2,415,251 to 2,416,312
_11285
Group
Condition
PS417
_11265
PS417
_11270
PS417
_11275
PS417
_11280
PS417
_11285
phage
P. simiae OR1 MOI 0.1
N.D.
N.D.
-1.2
-0.3
N.D.
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
-0.3
-1.0
N.D.
phage
JP1 MOI 1
N.D.
N.D.
-0.4
-0.7
N.D.
agar plate interaction
Taped volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
-0.7
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Methionine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.6
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.9
-0.2
N.D.
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
N.D.
-1.0
+0.3
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
-0.9
+0.4
N.D.
carbon source
L-Valine (C)
N.D.
N.D.
+0.4
-0.8
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
+0.4
-0.7
N.D.
soil
soil sample 5; outgrowth in LB
N.D.
N.D.
-0.7
+0.5
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
N.D.
N.D.
-0.6
+0.4
N.D.
seeds
Growth on radish seeds for 72 hours
N.D.
N.D.
-1.2
+1.0
N.D.
phage
P. simiae OR Antrim MOI 10
N.D.
N.D.
-0.6
+0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
-0.7
+0.7
N.D.
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
+0.5
-0.6
N.D.
carbon source
RCH2_defined_glucose with MS_media 1x
N.D.
N.D.
-0.5
+0.5
N.D.
supernatant
LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
N.D.
N.D.
-0.6
+0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.8
-0.5
N.D.
stress
TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
N.D.
N.D.
+0.7
-0.4
N.D.
phage
P. simiae OR Antrim MOI 10
N.D.
N.D.
-0.3
+0.8
N.D.
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
N.D.
N.D.
-0.2
+0.9
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.7
+0.3
N.D.
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
N.D.
N.D.
+0.3
+0.8
N.D.
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
N.D.
+0.3
+0.8
N.D.
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
+1.0
+0.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
+0.6
+0.6
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
N.D.
+0.3
+0.9
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
+1.0
+0.5
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
N.D.
N.D.
+1.4
+0.8
N.D.
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