Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_11265 and PS417_11270 overlap by 14 nucleotidesPS417_11270 and PS417_11275 are separated by 148 nucleotidesPS417_11275 and PS417_11280 are separated by 8 nucleotidesPS417_11280 and PS417_11285 overlap by 4 nucleotides PS417_11265: PS417_11265 - general secretion pathway protein GspC, at 2,412,995 to 2,413,426 _11265 PS417_11270: PS417_11270 - general secretion pathway protein GspI, at 2,413,413 to 2,413,778 _11270 PS417_11275: PS417_11275 - general secretion pathway protein GspG, at 2,413,927 to 2,414,376 _11275 PS417_11280: PS417_11280 - HxcX atypical pseudopilin, at 2,414,385 to 2,415,254 _11280 PS417_11285: PS417_11285 - general secretion pathway protein GspL, at 2,415,251 to 2,416,312 _11285
Group Condition PS417_11265 PS417_11270 PS417_11275 PS417_11280 PS417_11285
phage P. simiae OR1 MOI 0.1 N.D. N.D. -1.2 -0.3 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.3 -1.0 N.D.
phage JP1 MOI 1 N.D. N.D. -0.4 -0.7 N.D.
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.7 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Methionine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.6 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.9 -0.2 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. N.D. -1.0 +0.3 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -0.9 +0.4 N.D.
carbon source L-Valine (C) N.D. N.D. +0.4 -0.8 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.4 -0.7 N.D.
soil soil sample 5; outgrowth in LB N.D. N.D. -0.7 +0.5 N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. N.D. -0.6 +0.4 N.D.
seeds Growth on radish seeds for 72 hours N.D. N.D. -1.2 +1.0 N.D.
phage P. simiae OR Antrim MOI 10 N.D. N.D. -0.6 +0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.7 +0.7 N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. +0.5 -0.6 N.D.
carbon source RCH2_defined_glucose with MS_media 1x N.D. N.D. -0.5 +0.5 N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x N.D. N.D. -0.6 +0.6 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.8 -0.5 N.D.
stress TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 N.D. N.D. +0.7 -0.4 N.D.
phage P. simiae OR Antrim MOI 10 N.D. N.D. -0.3 +0.8 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. -0.2 +0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.7 +0.3 N.D.
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. N.D. +0.3 +0.8 N.D.
carbon source 2'-Deoxyinosine 5 mM (C) N.D. N.D. +0.3 +0.8 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +1.0 +0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.6 +0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +0.3 +0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. +1.0 +0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +1.4 +0.8 N.D.
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