Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_11205 and PS417_11210 are separated by 101 nucleotidesPS417_11210 and PS417_11215 are separated by 55 nucleotidesPS417_11215 and PS417_11220 are separated by 87 nucleotidesPS417_11220 and PS417_11225 overlap by 4 nucleotides PS417_11205: PS417_11205 - LysR family transcriptional regulator, at 2,403,221 to 2,404,096 _11205 PS417_11210: PS417_11210 - LysR family transcriptional regulator, at 2,404,198 to 2,404,584 _11210 PS417_11215: PS417_11215 - arylesterase, at 2,404,640 to 2,405,458 _11215 PS417_11220: PS417_11220 - signal peptide protein, at 2,405,546 to 2,406,937 _11220 PS417_11225: PS417_11225 - glyoxalase, at 2,406,934 to 2,407,830 _11225
Group Condition PS417_11205 PS417_11210 PS417_11215 PS417_11220 PS417_11225
phage JP1 MOI 10 N.D. N.D. -0.4 -0.3 -1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.9 -0.3 -0.8
phage Wcs_0.1 N.D. N.D. -0.7 -0.3 -0.8
phage Wcs_1 N.D. N.D. -0.6 -0.4 -0.4
no phage control Time6-nophage N.D. N.D. -0.7 -0.3 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.5 -0.4 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.1 -0.5 -0.8
liquid stress Fraxetin 2 mM; liquid stress N.D. N.D. -0.2 -0.2 -0.7
ecofab LB in EcoFAB_3.5mL N.D. N.D. -0.2 -0.1 -0.8
nophagecontrol Only library N.D. N.D. +0.0 +0.2 -1.3
carbon source 2'-Deoxyinosine 5 mM (C) N.D. N.D. +0.1 -0.2 -0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. +0.1 -0.4 -0.6
soil soil sample 6; outgrowth in LB N.D. N.D. -0.3 +0.3 -0.7
carbon source Carbon source D-Mannitol 10 mM N.D. N.D. -0.6 -0.4 +0.4
carbon source L-Valine (C) N.D. N.D. -0.5 +0.3 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.5 -0.5 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.7 -0.3 -0.9
phage JP1 MOI 1 N.D. N.D. -0.6 +0.4 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.2 +0.3 -0.6
seeds Growth on radish seeds for 24 hours N.D. N.D. +0.1 +0.3 -0.8
phage P. simiae OR1 MOI 0.1 N.D. N.D. -0.7 -0.4 +0.8
phage P. simiae Grant Run MOI 10 N.D. N.D. -0.1 -0.5 +0.6
seeds Growth on radish seeds for 72 hours N.D. N.D. +0.4 +0.3 -0.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.7 -0.0 -0.5
carbon source Trisodium citrate (C) N.D. N.D. -0.3 -0.1 +0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.3 -0.1 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +1.1 +0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +0.9 +0.3 -0.2
seeds Growth on radish seeds for 72 hours N.D. N.D. +0.5 +0.7 -0.1
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. N.D. +0.6 +0.2 +0.3
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