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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_11165 and PS417_11170 overlap by 4 nucleotides
PS417_11170 and PS417_11175 overlap by 14 nucleotides
PS417_11175 and PS417_11180 are separated by 173 nucleotides
PS417_11180 and PS417_11185 overlap by 4 nucleotides
PS417_11165: PS417_11165 - omega amino acid--pyruvate aminotransferase, at 2,394,487 to 2,395,782
_11165
PS417_11170: PS417_11170 - aldehyde dehydrogenase, at 2,395,779 to 2,397,200
_11170
PS417_11175: PS417_11175 - AraC family transcriptional regulator, at 2,397,187 to 2,398,125
_11175
PS417_11180: PS417_11180 - TetR family transcriptional regulator, at 2,398,299 to 2,398,928
_11180
PS417_11185: PS417_11185 - dimethylallyltransferase, at 2,398,925 to 2,399,602
_11185
Group
Condition
PS417
_11165
PS417
_11170
PS417
_11175
PS417
_11180
PS417
_11185
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-0.0
-1.6
-1.0
-1.3
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x
N.D.
+0.3
-1.7
-1.6
-0.4
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
N.D.
-0.1
-1.5
-0.9
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.2
-0.8
-0.9
-0.6
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-0.0
-0.9
-1.0
-0.5
soil
soil sample 8; outgrowth in LB
N.D.
+0.8
-0.9
-1.9
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.5
-1.5
-0.5
+0.2
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
+0.0
-0.8
-0.2
-1.3
supernatant
Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 1x
N.D.
+0.2
-1.9
-0.3
-0.2
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.1x
N.D.
+0.2
-0.9
-1.0
-0.4
carbon source
RCH2_defined_glucose with MS_media 1x
N.D.
+0.1
-1.1
-0.6
-0.5
seeds
Growth on radish seeds for 24 hours
N.D.
+0.2
-1.7
-0.9
+0.6
phage
Wcs_1
N.D.
-0.5
-0.4
+0.3
-1.2
phage
JP1 MOI 0.1
N.D.
+0.3
-1.4
-0.1
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.2
-1.4
+0.1
+0.6
soil
soil sample 2; outgrowth in LB
N.D.
+0.9
-1.4
-0.1
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.5
-1.5
-0.4
+0.6
seeds
Growth on radish seeds for 72 hours
N.D.
-0.2
-1.1
-0.2
+1.1
rhizosphere
rhizosphere sample 8; outgrowth in LB
N.D.
+1.0
-0.4
-0.7
-0.2
carbon source
Carbon source L-Ornithine 10 mM
N.D.
+0.1
-1.1
+0.5
+0.7
seeds
Growth on radish seeds for 72 hours
N.D.
+0.1
-0.0
-1.0
+1.1
rhizosphere
rhizosphere sample 1; outgrowth in LB
N.D.
+1.3
+0.4
-0.2
-0.6
soil
soil sample 7; outgrowth in LB
N.D.
+0.9
+0.8
-0.3
-0.3
phage
JP1 MOI 10
N.D.
+0.5
-0.4
+0.6
+1.3
phage
P. simiae OR Antrim MOI 0.1
N.D.
-0.1
+0.6
+1.2
+0.4
carbon source
2'-Deoxyinosine 5 mM (C)
N.D.
+0.2
+0.9
+0.7
+0.5
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
+0.3
+2.5
+0.4
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.4
+0.2
+0.9
+1.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
+0.5
-0.0
+0.2
+2.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+0.6
+1.5
+0.4
+1.4
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