Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_11105 and PS417_11110 are separated by 1 nucleotidesPS417_11110 and PS417_11115 are separated by 50 nucleotidesPS417_11115 and PS417_11120 are separated by 74 nucleotidesPS417_11120 and PS417_11125 are separated by 180 nucleotides PS417_11105: PS417_11105 - pyridine nucleotide-disulfide oxidoreductase, at 2,380,476 to 2,381,675 _11105 PS417_11110: PS417_11110 - xylose isomerase, at 2,381,677 to 2,382,495 _11110 PS417_11115: PS417_11115 - peroxidase, at 2,382,546 to 2,383,502 _11115 PS417_11120: PS417_11120 - UDP pyrophosphate phosphatase, at 2,383,577 to 2,384,446 _11120 PS417_11125: PS417_11125 - hypothetical protein, at 2,384,627 to 2,385,133 _11125
Group Condition PS417_11105 PS417_11110 PS417_11115 PS417_11120 PS417_11125
soil soil sample 4; outgrowth in LB N.D. -0.5 -0.3 -1.0 +0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.3 -0.6 -0.7 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 -0.2 -0.3 -1.0
phage JP1 MOI 10 N.D. -0.8 +0.3 +0.1 -0.6
liquid stress Fraxetin 3 mM; liquid stress N.D. -0.2 -0.3 +0.4 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 -0.8 +0.3 -0.3
stress R2A with Fusaric 120 ug/mL N.D. -0.4 +0.2 +0.4 -0.8
nophagecontrol Only library N.D. -0.1 +0.1 +0.5 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 +0.4 +0.2 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.3 -0.7 +0.3 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.3 +0.6 +0.0 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.6 +0.3 -0.7 -0.6
phage P. simiae Grant Run MOI 0.1 N.D. +0.6 -0.2 -0.6 -0.2
carbon source L-Leucine (C) N.D. -0.5 +0.2 +0.5 -0.5
phage JP1 MOI 10 N.D. -0.4 +0.3 +0.3 -0.5
motility_chemotaxis Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.4 +0.3 -0.0 -0.9
seeds Growth on radish seeds for 24 hours N.D. +0.7 -0.3 +0.4 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.7 +0.7 +0.3 -0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.5 -0.1 -1.2 +0.9
stress methylglyoxal 0.032 vol% N.D. +0.4 -0.5 -0.3 +0.6
seeds Growth on radish seeds for 72 hours N.D. +1.1 -0.1 -0.2 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.2 +0.6 +0.1 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.7 -0.4 +0.4 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.5 +0.4 +0.6 -0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.3 +0.3 +0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 -0.3 +0.4 +1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 +0.6 +0.8 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.2 +0.2 +1.3 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.1 +0.6 +0.4 +0.4
carbon source L-Valine (C) N.D. +0.3 +0.6 +0.8 +0.2
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