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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_10955 and PS417_10960 are separated by 57 nucleotides
PS417_10960 and PS417_10965 are separated by 210 nucleotides
PS417_10965 and PS417_10970 are separated by 145 nucleotides
PS417_10970 and PS417_10980 are separated by 423 nucleotides
PS417_10955: PS417_10955 - porin, at 2,344,237 to 2,345,502
_10955
PS417_10960: PS417_10960 - quinate dehydrogenase, at 2,345,560 to 2,347,968
_10960
PS417_10965: PS417_10965 - chemotaxis protein, at 2,348,179 to 2,350,122
_10965
PS417_10970: PS417_10970 - effector protein, at 2,350,268 to 2,351,158
_10970
PS417_10980: PS417_10980 - ArsR family transcriptional regulator, at 2,351,582 to 2,351,881
_10980
Group
Condition
PS417
_10955
PS417
_10960
PS417
_10965
PS417
_10970
PS417
_10980
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
-0.0
-4.9
-0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
+0.5
-3.5
-0.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-0.4
-1.1
-0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.2
-0.2
-0.3
+0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.0
-1.3
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
-0.3
-0.3
-0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.0
-0.3
+0.1
-0.3
N.D.
motility
inner cut, LB soft agar motility assay
-0.5
-0.4
-0.1
-0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.2
-0.2
-0.0
+0.2
N.D.
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-1.1
-0.3
+0.1
+0.2
N.D.
stress
Doxycycline hyclate 0.001 mg/ml
-1.3
+0.1
+0.2
+0.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.0
-0.1
-0.1
+0.4
N.D.
stress
methylglyoxal 0.032 vol%
-1.0
-0.1
+0.3
+0.2
N.D.
phage
Wcs_0.1
-0.9
+0.3
+0.1
-0.1
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.8
+0.2
+0.3
+0.2
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
+0.7
-0.3
-0.1
-0.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
+0.4
+0.6
-0.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.3
+0.6
+0.4
-0.5
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.1
-0.2
-0.0
-0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.8
-0.1
-0.2
+0.2
N.D.
phage
P. simiae ORA MOI 1
+0.7
+0.0
-0.3
+0.2
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.5
+0.2
-0.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
+0.2
-0.3
+0.5
N.D.
phage
P. simiae OR Antrim MOI 0.1
+0.6
+0.2
-0.0
+0.4
N.D.
soil
soil sample 2; outgrowth in LB
+0.7
+0.5
+0.1
+0.0
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.6
-0.1
+0.6
+0.3
N.D.
carbon source
2'-Deoxyinosine 5 mM (C)
+0.7
+0.3
+0.2
+0.1
N.D.
soil
soil sample 6; outgrowth in LB
+0.6
+0.4
+0.2
+0.2
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+1.0
+0.2
-0.0
+0.3
N.D.
soil
soil sample 4; outgrowth in LB
+1.1
+0.5
+0.7
-0.1
N.D.
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