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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_10870 and PS417_10875 overlap by 4 nucleotides
PS417_10875 and PS417_10880 are separated by 2 nucleotides
PS417_10880 and PS417_10885 are separated by 16 nucleotides
PS417_10885 and PS417_10890 overlap by 1 nucleotides
PS417_10870: PS417_10870 - GABA permease, at 2,322,728 to 2,324,137
_10870
PS417_10875: PS417_10875 - AraC family transcriptional regulator, at 2,324,134 to 2,325,210
_10875
PS417_10880: PS417_10880 - cytochrome c component of deoxyribose dehydrogenase
(from data)
, at 2,325,213 to 2,326,556
_10880
PS417_10885: PS417_10885 - Deoxyribose 1-dehydrogenase, beta subunit
(from data)
, at 2,326,573 to 2,328,792
_10885
PS417_10890: PS417_10890 - Deoxyribose 1-dehydrogenase, alpha subunit
(from data)
, at 2,328,792 to 2,329,247
_10890
Group
Condition
PS417
_10870
PS417
_10875
PS417
_10880
PS417
_10885
PS417
_10890
carbon source
2-Deoxy-D-Ribose (C)
-0.3
+0.1
-1.4
-3.9
-4.8
carbon source
2-Deoxy-D-Ribose (C)
+0.4
+0.1
-1.4
-4.7
-4.3
carbon source
2-Deoxy-D-Ribose 10 mM (C)
-0.1
+0.1
-1.6
-3.9
-2.9
carbon source
2-Deoxy-D-Ribose 10 mM (C)
+0.8
+0.1
-1.9
-3.0
-3.3
carbon source
2-Deoxy-D-Ribose (C)
-0.0
+0.3
-0.8
-2.2
-2.4
carbon source
2-Deoxy-D-Ribose (C)
-0.1
+0.1
-0.9
-2.3
-1.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.1
-0.2
-0.5
+0.1
+0.1
stress
2-Furfuraldehyde 0.03 vol%
+0.4
+0.1
-0.9
-1.0
-0.8
carbon source
2'-Deoxyinosine 5 mM (C)
-0.4
-0.3
-0.9
-0.5
-0.1
stress
2-Furfuraldehyde 0.06 vol%
+0.4
+0.0
-0.6
-0.8
-1.0
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-1.0
+0.1
-0.6
-0.3
-0.1
phage
P. simiae OR1 MOI 0.1
-1.7
-0.1
-0.0
-0.1
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.2
+0.1
+0.1
-0.1
-0.4
agar plate interaction control
Taped volatile agar plate with no fungus
-1.1
-0.5
+0.3
-0.7
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.7
-0.2
-0.4
-0.4
+0.2
agar plate interaction control
Taped volatile agar plate with no fungus
-0.8
-0.2
-0.6
-0.0
+0.2
seeds
Growth on radish seeds for 72 hours
-1.1
+0.1
+0.1
-0.4
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-1.0
-0.3
+0.2
-0.1
+0.1
soil
soil sample 6; outgrowth in LB
-0.9
-0.1
-0.6
+0.2
+0.5
phage
P. simiae OR Antrim MOI 10
-0.6
-0.2
+0.7
+0.0
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.3
-0.6
+0.5
-0.4
seeds
Growth on radish seeds for 72 hours
-0.1
+1.3
-0.7
-0.4
-0.5
motility
inner cut, LB soft agar motility assay
+0.6
+0.1
-0.4
+0.2
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.0
+0.5
-0.2
-0.5
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.6
+0.3
+0.5
-0.3
-0.4
rhizosphere
rhizosphere sample 1; outgrowth in LB
+0.6
-0.4
+0.2
+0.3
+0.5
phage
P. simiae ORA MOI 0.1
+0.9
-0.2
+0.6
+0.0
+0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.1
+0.4
-0.1
+0.1
+0.2
seeds
Growth on radish seeds for 72 hours
-0.4
+0.6
+0.6
+0.6
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.1
+0.4
+0.2
+0.4
+0.1
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