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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_10825 and PS417_10830 are separated by 6 nucleotides
PS417_10830 and PS417_10835 are separated by 136 nucleotides
PS417_10835 and PS417_10840 are separated by 19 nucleotides
PS417_10840 and PS417_10845 overlap by 12 nucleotides
PS417_10825: PS417_10825 - glycine/betaine ABC transporter substrate-binding protein, at 2,314,492 to 2,315,391
_10825
PS417_10830: PS417_10830 - LysR family transcriptional regulator, at 2,315,398 to 2,316,315
_10830
PS417_10835: PS417_10835 - major facilitator transporter, at 2,316,452 to 2,317,768
_10835
PS417_10840: PS417_10840 - amidase, at 2,317,788 to 2,318,561
_10840
PS417_10845: PS417_10845 - hypothetical protein, at 2,318,550 to 2,318,858
_10845
Group
Condition
PS417
_10825
PS417
_10830
PS417
_10835
PS417
_10840
PS417
_10845
stress
Phosphomycin 0.001 mg/ml
-0.7
N.D.
-0.1
-0.5
N.D.
phage
JP1 MOI 0.1
-0.5
N.D.
-0.4
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
N.D.
-0.5
-1.4
N.D.
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x
-0.2
N.D.
-0.3
-0.7
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.3
N.D.
+0.1
-0.8
N.D.
exudates
RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x
+0.2
N.D.
-0.4
-0.8
N.D.
agar plate interaction control
Taped volatile agar plate with no fungus
+0.1
N.D.
-0.0
-1.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.2
N.D.
+0.1
-1.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
-0.2
-0.8
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
-0.2
-0.9
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
+0.0
-1.1
N.D.
solid stress
Fraxetin 1 mM; solid stress
+0.3
N.D.
+0.1
-0.7
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.6
N.D.
-0.2
-0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
+0.2
-0.6
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
N.D.
+0.2
+0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.6
N.D.
+0.2
-0.5
N.D.
soil
soil sample 4; outgrowth in LB
-0.1
N.D.
-0.4
+1.0
N.D.
phage
Wcs_1
+0.0
N.D.
-0.3
+0.9
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.9
N.D.
+0.2
-0.2
N.D.
carbon source
L-Valine (C)
+0.8
N.D.
-0.1
+0.3
N.D.
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.3
N.D.
+0.4
+0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.7
N.D.
-0.1
+0.5
N.D.
phage
P. simiae Grant Run MOI 0.1
+0.1
N.D.
-0.4
+1.4
N.D.
carbon source
octanoate (C)
+0.2
N.D.
+0.0
+0.9
N.D.
supernatant control
Vogels_fungal_media 0.1X
+0.9
N.D.
-0.0
+0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
+0.3
+0.3
N.D.
seeds
Growth on radish seeds for 72 hours
-0.2
N.D.
+0.3
+1.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
N.D.
+0.2
+0.5
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
N.D.
+0.2
+0.6
N.D.
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
+0.5
N.D.
+0.1
+0.8
N.D.
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